Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed paraffin-embedded tissue specimens

摘要

BackgroundOver the past five years in situ hybridization techniques employing tyramide amplification reagents have been developed and promise the potential detection of low/single-copy nucleic acid sequences. However the increased sensitivity that tyramide amplification brings about may also lead to problems of background staining that confound data interpretation.