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Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping

机译：有效生成经典猪瘟病毒众多全长cDNA克隆以进行单倍型分析的策略

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BackgroundDirect molecular cloning of full-length cDNAs derived from viral RNA is an approach to identify the individual viral genomes within a virus population. This enables characterization of distinct viral haplotypes present during infection.

机译：背景技术直接克隆分子自病毒RNA的全长cDNA是一种鉴定病毒种群内单个病毒基因组的方法。这使得能够表征感染期间存在的不同病毒单倍型。

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期刊名称 BMC Genomics
作者
Camille Melissa Johnston; Ulrik Fahnøe; Graham J. Belsham; Thomas Bruun Rasmussen;
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作者单位

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年(卷),期 2018(19),-1
年度 2018
页码 600
总页数 9
原文格式 PDF
正文语种
中图分类遗传学;应用微生物学;
关键词
RNA Genome Bacterial artificial chromosome RNA virus Pestivirus Haplotyping;

机译：RNA;基因组;细菌人工染色体;RNA病毒;瘟病毒;单体型;

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专利

1. Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping [J] . Camille Melissa Johnston, Ulrik Fahn?e, Graham J. Belsham, BMC Genomics . 2018,第1期

机译：典型猪瘟病毒众多全长cDNA克隆的策略进行单倍型
2. Recovery of infectious classical swine fever virus (CSFV) from full-length genomic cDNA clones by a swine kidney cell line expressing bacteriophage T7 RNA polymerase. [J] . van Gennip-HG, van Rijn-PA, Widjojoatmodjo MN, Journal of Virological Methods . 1999,第1a2期

机译：通过表达噬菌体T7 RNA聚合酶的猪肾细胞系从全长基因组cDNA克隆中回收感染性经典猪瘟病毒（CSFV）。
3. Sequencing and rescuing a highly virulent classical swine fever virus: Chinese strain cF114 from a full-length cDNA clone [J] . NIE Yuchun, CHEN Jianguo, DING Mingxiao Chinese science bulletin . 2003,第11期

机译：测序和拯救强毒的经典猪瘟病毒：来自全长cDNA克隆的中国菌株cF114
4. GENERATION AND EFFICACY ASSESSMENT OF A CHIMERIC ANTIGEN E2-CD154 AS A MARKER CLASSICAL SWINE FEVER VIRUS SUBUNIT VACCINE PRODUCED IN HEK 293 AND CHO K1 MAMMALIAN CELLS [C] . Lidice Mendez-Perez, Elianet Lorenzo, Elsa M. Rodriguez, Conference on vaccine technology VI . 2018

机译：嵌合抗原E2-CD154作为HEK 293和CHO K1哺乳动物细胞中标记的经典猪瘟病毒亚单位疫苗的产生和功效评估
5. Generation of full-length cDNA clone and functional analysis of leader proteases of Grapevine leafroll-associated virus-2. [D] . Liu, Yu-Ping. 2009

机译：葡萄叶片相关病毒2的全长cDNA克隆的产生和前导蛋白酶的功能分析。
6. Nucleotide sequence of classical swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA. [O] . N Ruggli, J D Tratschin, C Mittelholzer, 1996

机译：经典猪瘟病毒株Alfort / 187的核苷酸序列和稳定克隆的全长cDNA的感染性RNA的转录。
7. Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic defective interfering particles [O] . G Meyers, H J Thiel, T Rümenapf 1996

机译：古典猪瘟病毒：从cDNA构建体中恢复传染性病毒，并产生重组细胞质大缺陷干扰颗粒

Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping

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