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Identification of stable quantitative trait loci (QTLs) for fiber quality traits across multiple environments in Gossypium hirsutum recombinant inbred line population

机译:陆地棉重组自交系群体中多种环境中纤维品质性状的稳定数量性状基因座(QTL)的鉴定

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摘要

BackgroundThe identification of quantitative trait loci (QTLs) that are stable and consistent across multiple environments and populations plays an essential role in marker-assisted selection (MAS). In the present study, we used 28,861 simple sequence repeat (SSR) markers, which included 12,560 Gossypium raimondii (D genome) sequence-based SSR markers to identify polymorphism between two upland cotton strains 0–153 and sGK9708. A total of 851 polymorphic primers were finally selected and used to genotype 196 recombinant inbred lines (RIL) derived from a cross between 0 and 153 and sGK9708 and used to construct a linkage map. The RIL population was evaluated for fiber quality traits in six locations in China for five years. Stable QTLs identified in this intraspecific cross could be used in future cotton breeding program and with fewer obstacles.
机译:背景技术在多个环境和人群中稳定且一致的定量性状基因座(QTL)的鉴定在标记辅助选择(MAS)中起着至关重要的作用。在本研究中,我们使用了28,861个简单序列重复(SSR)标记,其中包括12,560个雷蒙氏棉(D基因组)基于序列的SSR标记,以鉴定两个陆地棉0–153和sGK9708菌株之间的多态性。最终选择了总共851个多态性引物,并用于对196个重组自交系(RIL)进行基因分型,该自交系来自0和153与sGK9708之间的杂交,并用于构建连锁图。连续五年在中国六个地区对RIL人群的纤维质量特征进行了评估。在种内杂交中鉴定出的稳定QTL可以用于未来的棉花育种计划,且障碍较少。

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