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Effect of ensiled mulberry leaves and sun-dried mulberry fruit pomace on the fecal bacterial community composition in finishing steers

机译:蚕桑叶和晒果桑果渣对育肥牛粪中细菌群落组成的影响

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摘要

BackgroundHere, we aimed to investigate the effects of ensiled mulberry leaves (EML) and sun-dried mulberry fruit pomace (SMFP) on fecal bacterial communities in Simmental crossbred finishing steers. To this end, the steers were reared on a standard TMR diet, standard diet containing EML, and standard diet containing SMFP. The protein and energy levels of all the diets were similar. Illumina MiSeq sequencing of the V4 region of the 16S rRNA gene and quantitative real-time PCR were used to analyze and detect the fecal bacterial community.
机译:背景技术在此,我们旨在研究青贮桑叶(EML)和晒干桑果渣(SMFP)对西门塔尔杂交育种公牛粪便细菌群落的影响。为此,将ste牛饲养在标准TMR日粮,含EML的标准日粮和含SMFP的标准日粮中。所有饮食的蛋白质和能量水平相似。使用Illumina MiSeq对16S rRNA基因的V4区进行测序和定量实时PCR来分析和检测粪便细菌群落。

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