Reverse transcription-quantitative polymerase chain reaction: description of a RIN-based algorithm for accurate data normalization

摘要

BackgroundReverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold standard technique for mRNA quantification, but appropriate normalization is required to obtain reliable data. Normalization to accurately quantitated RNA has been proposed as the most reliable method for in vivo biopsies. However, this approach does not correct differences in RNA integrity.