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Comparative transcriptomics analysis of compatible wild type and incompatible ΔlaeA mutant strains of Epichloë festucae in association with perennial ryegrass

机译:多年生黑麦草与表皮草的兼容野生型和不兼容ΔlaeA突变株的比较转录组学分析

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摘要

Epichloë festucae fungi form bioprotective endophytic symbioses with perennial ryegrass. Although this interaction is economically important, relatively little is known about the molecular processes and regulatory genes that are involved in establishing a compatible symbiosis. The present study utilised next-generation sequencing to investigate the genes required for establishing a compatible symbiotic interaction between E. festucae and perennial ryegrass. A comparative transcriptomics study, comparing the compatible symbiotic interaction of E. festucae/perennial ryegrass with the incompatible interaction of a ΔlaeA mutant strain of E.festucae/perennial ryegrass, was performed two weeks after inoculation. Differentially expressed genes were identified and classified according to gene ontology and functional annotation analyses. The raw data of this study have been deposited at SRA database with the BioProject ID PRJNA513830.
机译:羊茅真菌与多年生黑麦草形成生物保护性内生共生酶。尽管这种相互作用在经济上很重要,但对建立相容共生所涉及的分子过程和调控基因知之甚少。本研究利用下一代测序技术来研究在鸡白茅和多年生黑麦草之间建立相容的共生相互作用所需的基因。接种两周后,进行了一项比较转录组学研究,比较了马贞木/多年生黑麦草的相容共生相互作用与马蹄莲/多年生黑麦草的ΔlaeA突变株的不相容相互作用。根据基因本体论和功能注释分析鉴定和分类差异表达的基因。这项研究的原始数据已以BioProject ID PRJNA513830的形式保存在SRA数据库中。

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