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Experimental data from flesh quality assessment and shelf life monitoring of high pressure processed European sea bass (Dicentrarchus labrax) fillets

机译:来自高压加工的欧洲鲈鱼(Dicentrarchus labrax)鱼片肉质评估和保质期监测的实验数据

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摘要

Fresh fish are highly perishable food products and their short shelf-life limits their commercial exploitation and leads to waste, which has a negative impact on aquaculture sustainability. New non-thermal food processing methods, such as high pressure (HP) processing, prolong shelf-life while assuring high food quality. The effect of HP processing (600MPa, 25 °C, 5min) on European sea bass (Dicentrarchus labrax) fillet quality and shelf life was investigated. The data presented comprises microbiome and proteome profiles of control and HP-processed sea bass fillets from 1 to 67 days of isothermal storage at 2 °C. Bacterial diversity was analysed by Illumina high-throughput sequencing of the 16S rRNA gene in pooled DNAs from control or HP-processed fillets after 1, 11 or 67 days and the raw reads were deposited in the NCBI-SRA database with accession number PRJNA517618. Yeast and fungi diversity were analysed by high-throughput sequencing of the internal transcribed spacer (ITS) region for control and HP-processed fillets at the end of storage (11 or 67 days, respectively) and have the SRA accession number PRJNA517779. Quantitative label-free proteomics profiles were analysed by SWATH-MS (Sequential Windowed data independent Acquisition of the Total High-resolution-Mass Spectra) in myofibrillar or sarcoplasmic enriched protein extracts pooled for control or HP-processed fillets after 1, 11 and 67 days of storage. Proteome data was deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD012737. These data support the findings reported in the associated manuscript “High pressure processing of European sea bass (Dicentrarchus labrax) fillets and tools for flesh quality and shelf life monitoring”, Tsironi et al., 2019, JFE 262:83–91, doi.org/10.1016/j.jfoodeng.2019.05.010.
机译:鲜鱼是高度易腐烂的食品,其保质期短限制了其商业开发并导致浪费,这对水产养殖业的可持续性产生了负面影响。新型非热食品加工方法,例如高压(HP)加工,可延长保质期,同时确保高食品质量。研究了高压加工(600MPa,25°C,5min)对欧洲鲈鱼(Dicentrarchus labrax)的鱼片质量和保质期的影响。呈现的数据包括在2°C下从1到67天等温储存的对照和HP处理的鲈鱼鱼片的微生物组和蛋白质组概况。在1、11或67天后,通过Illumina高通量测序从对照鱼片或HP处理鱼片中收集的DNA中的16S rRNA基因,分析细菌的多样性,并将原始读数保存在NCBI-SRA数据库中,登录号为PRJNA517618。在存储结束时(分别为11天或67天),通过内部转录间隔区(ITS)用于对照和HP处理的鱼片的高通量测序,分析了酵母和真菌的多样性,其SRA登录号为PRJNA517779。 1天,11天和67天后,通过汇集在肌原纤维或肌浆丰富蛋白提取物中的SWATH-MS(独立于总高分辨率质谱的连续窗口数据采集)分析了无标记的蛋白质组学概况的存储。蛋白质组数据通过PRIDE合作伙伴存储库存储在ProteomeXchange联盟中,数据集标识符为PXD012737。这些数据支持相关手稿``欧洲海鲈鱼片的高压加工和肉质和货架期监控工具''中的发现,Tsironi等人,2019年,JFE 262:83-91,doi。 org / 10.1016 / j.jfoodeng.2019.05.010。

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