Stoichiometry of the Photosynthetic Apparatus and Phycobilisome Structure of the Cyanobacterium Plectonema boryanum UTEX 485 Are Regulated by Both Light and Temperature

摘要

The role of growth temperature and growth irradiance on the regulation of the stoichiometry and function of the photosynthetic apparatus was examined in the cyanobacterium Plectonema boryanum UTEX 485 by comparing mid-log phase cultures grown at either 29°C/150 μmol m⁻² s⁻¹, 29°C/750 μmol m⁻² s⁻¹, 15°C/150 μmol m⁻² s⁻¹, or 15°C/10 μmol m⁻² s⁻¹. Cultures grown at 29°C/750 μmol m⁻² s⁻¹ were structurally and functionally similar to those grown at 15°C/150 μmol m⁻² s⁻¹, whereas cultures grown at 29°C/150 μmol m⁻² s⁻¹ were structurally and functionally similar to those grown at 15°C/10 μmol m⁻² s⁻¹. The stoichiometry of specific components of the photosynthetic apparatus, such as the ratio of photosystem (PS) I to PSII, phycobilisome size and the relative abundance of the cytochrome b6/f complex, the plastoquinone pool size, and the NAD(P)H dehydrogenase complex were regulated by both growth temperature and growth irradiance in a similar manner. This indicates that temperature and irradiance may share a common sensing/signaling pathway to regulate the stoichiometry and function of the photosynthetic apparatus in P. boryanum. In contrast, the accumulation of neither the D1 polypeptide of PSII, the large subunit of Rubisco, nor the CF1 α-subunit appeared to be regulated by the same mechanism. Measurements of P700 photooxidation in vivo in the presence and absence of inhibitors of photosynthetic electron transport coupled with immunoblots of the NAD(P)H dehydrogenase complex in cells grown at either 29°C/750 μmol m⁻² s⁻¹ or 15°C/150 μmol m⁻² s⁻¹ are consistent with an increased flow of respiratory electrons into the photosynthetic intersystem electron transport chain maintaining P700 in a reduced state relative to cells grown at either 29°C/150 μmol m⁻² s⁻¹ or 15°C/10 μmol m⁻² s⁻¹. These results are discussed in terms of acclimation to excitation pressure imposed by either low growth temperature or high growth irradiance.