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Y3IP1 a Nucleus-Encoded Thylakoid Protein Cooperates with the Plastid-Encoded Ycf3 Protein in Photosystem I Assembly of Tobacco and Arabidopsis

机译:Y3IP1一种核编码类囊体蛋白与烟草和拟南芥光系统I装配中的质体编码Ycf3蛋白协同作用

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摘要

The intricate assembly of photosystem I (PSI), a large multiprotein complex in the thylakoid membrane, depends on auxiliary protein factors. One of the essential assembly factors for PSI is encoded by ycf3 (hypothetical chloroplast reading frame number 3) in the chloroplast genome of algae and higher plants. To identify novel factors involved in PSI assembly, we constructed an epitope-tagged version of ycf3 from tobacco (Nicotiana tabacum) and introduced it into the tobacco chloroplast genome by genetic transformation. Immunoaffinity purification of Ycf3 complexes from the transplastomic plants identified a novel nucleus-encoded thylakoid protein, Y3IP1 (for Ycf3-interacting protein 1), that specifically interacts with the Ycf3 protein. Subsequent reverse genetics analysis of Y3IP1 function in tobacco and Arabidopsis thaliana revealed that knockdown of Y3IP1 leads to a specific deficiency in PSI but does not result in loss of Ycf3. Our data indicate that Y3IP1 represents a novel factor for PSI biogenesis that cooperates with the plastid genome-encoded Ycf3 in the assembly of stable PSI units in the thylakoid membrane.
机译:光系统I(PSI)的复杂组装是类囊体膜中的大型多蛋白复合物,取决于辅助蛋白因子。 PSI的重要装配因子之一是由藻类和高等植物的叶绿体基因组中的ycf3(假设的叶绿体阅读框编号3)编码的。为了确定参与PSI组装的新因素,我们从烟草(Nicotiana tabacum)构建了表位标记的ycf3,并通过遗传转化将其引入到烟草叶绿体基因组中。从转基因组植物中免疫亲和纯化Ycf3复合物,鉴定出一种新型的核编码类囊体蛋白Y3IP1(用于与Ycf3相互作用的蛋白1),该蛋白与Ycf3蛋白特异性相互作用。随后在烟草和拟南芥中对Y3IP1功能的反向遗传学分析显示,敲除Y3IP1会导致PSI的特定缺陷,但不会导致Ycf3的损失。我们的数据表明,Y3IP1代表PSI生物发生的新因素,它与类囊体膜中稳定PSI单元的装配中与质体基因组编码的Ycf3协同作用。

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