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Limitations progress and prospects of application of biotechnological tools in improvement of bamboo—a plant with extraordinary qualities

机译:生物技术工具在竹子改良中的局限性进展和应用前景

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摘要

Bamboos (family Poaceae) are the most beautiful and useful plants on the Earth, mainly found in the tropical and sub-tropical regions of the world. Bamboos are fast growing and early maturing, but lack of proper management of bamboo resources is leading to rapid reduction of the existing bamboosetum. Bamboo propagation through seeds is limited due to long flowering cycle of upto 120 years, seed sterility and short seed viability. Infrequent and unpredictable flowering events coupled with peculiar monocarpic behaviour i.e. flowering once before culm death, and extensive genome polyploidization are additional challenges for this woody group. Similarly, vegetative propagation by cuttings, offsets and rhizomes are also inadequate to cope up with the demand of planting stock due to large propagule size, limited availability, seasonal dependence, low multiplication rate and rooting percentage. Therefore, attempts have been made to propagate bamboos through in vitro techniques. In vitro flowering has also been achieved successfully in some bamboo species. Classification systems proposed to date need further support, as taxonomic delineation at lower levels is still lacking sufficient resolution. Tremendous advancement in molecular markers holds the promise to address the needs of bamboo taxonomy (systematics and identification) and diversity studies. Successful application of molecular marker techniques has been achieved in several bamboo species although, more studies are required to understand the population structure and genetic diversity of bamboos in a better way. In addition, some efforts have also been made to clone important genes from bamboos and also for genetic transformation using Agrobacterium and particle bombardment methods. An overview of the recent developments made in improvement of bamboos through in vitro propagation, molecular marker technologies, cloning, and transformation and transgenics has been presented. The future potential of improvement of bamboos using modern biotechnological tools has also been discussed.
机译:竹子(禾本科)是地球上最美丽,最有用的植物,主要​​存在于世界热带和亚热带地区。竹子生长迅速且早熟,但是缺乏适当的竹子资源管理导致现有竹子的迅速减少。竹子通过种子的繁殖受到限制,这是由于长达120年的长开花周期,种子不育和种子生存力短。这种木本植物群体还面临着罕见的,不可预测的开花事件以及独特的单掌行为,即在茎秆死亡前开花一次,以及广泛的基因组多倍体化。同样,由于繁殖体大,繁殖力有限,季节性依赖,繁殖率低和生根率低,由插枝,偏移和根状茎进行的营养繁殖也不足以满足种植种群的需求。因此,已经尝试通过体外技术繁殖竹子。在某些竹种中,体外开花也已成功实现。迄今为止提议的分类系统需要进一步的支持,因为较低级别的生物分类划界仍然缺乏足够的分辨率。分子标记的巨大进步有望满足竹类分类学(系统学和鉴定学)和多样性研究的需求。分子标记技术已在几种竹子中成功应用,尽管还需要进行更多的研究才能更好地了解竹子的种群结构和遗传多样性。另外,还进行了一些努力以从竹子中克隆重要的基因,以及使用农杆菌和粒子轰击方法进行遗传转化。概述了通过体外繁殖,分子标记技术,克隆,转化和转基因改良竹子的最新进展。还讨论了使用现代生物技术工具改良竹子的未来潜力。

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