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首页> 美国卫生研究院文献>Genetics >Multiple Roles of the Cox20 Chaperone in Assembly of Saccharomyces cerevisiae Cytochrome c Oxidase
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Multiple Roles of the Cox20 Chaperone in Assembly of Saccharomyces cerevisiae Cytochrome c Oxidase

机译：Cox20伴侣在酿酒酵母细胞色素c氧化酶组装中的多重作用。

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The subunit of Saccharomyces cerevisiae cytochrome c oxidase is synthesized in the mitochondrial matrix as a precursor whose leader peptide is rapidly processed by the inner membrane protease following translocation to the intermembrane space. Processing is chaperoned by , an integral inner membrane protein whose hydrophilic domains are located in the intermembrane space, and remains associated with mature, unassembled . The C-tail domain is exported post-translationally by the highly conserved translocase and associated proteins. We have found that is required for efficient export of the C-tail. Furthermore, interacts by co-immune precipitation with , and this interaction requires the presence of . We therefore propose that binding to on the trans side of the inner membrane accelerates dissociation of newly exported from the translocase, promoting efficient cycling of the translocase. The requirement for in cytochrome c oxidase assembly and respiratory growth is partially bypassed by href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000006228" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">yme1, href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000000549" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">mgr1 or href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000004721" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">mgr3 mutations, each of which reduce i-AAA protease activity in the intermembrane space. Thus, href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000002639" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Cox20 also appears to stabilize unassembled href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000007281" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Cox2 against degradation by the i-AAA protease. Pre-href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000007281" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Cox2 leader peptide processing by href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000004758" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Imp1 occurs in the absence of href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000002639" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Cox20 and i-AAA protease activity, but is greatly reduced in efficiency. Under these conditions some mature href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000007281" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Cox2 is assembled into cytochrome c oxidase allowing weak respiratory growth. Thus, the href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000002639" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Cox20 chaperone has important roles in leader peptide processing, C-tail export, and stabilization of href="http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000007281" data-ga-action="click_feat_suppl" ref="reftype=extlink&article-id=3276622&issue-id=205430&journal-id=301&FROM=Article%7CFront%20Matter&TO=External%7CLink%7CURI" target="_blank">Cox2.
机译：酿酒酵母细胞色素c氧化酶的亚基在线粒体基质中作为前体合成，其前导肽在转移到膜间空间后被内膜蛋白酶迅速加工。加工过程是由一个完整的内膜蛋白进行的，该蛋白的亲水结构域位于膜间空间，并与成熟的未组装蛋白结合。 C-尾结构域通过高度保守的转位酶和相关蛋白在翻译后输出。我们发现，这是有效出口C型尾翼所必需的。此外，通过与共免疫沉淀相互作用，并且这种相互作用需要的存在。因此，我们提出结合在内膜的反式上会加速新的从转位酶输出的解离，从而促进了转位酶的有效循环。 href =“ http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000006228” data-ga-action =“ click_feat_suppl”部分绕过了细胞色素C氧化酶组装和呼吸生长的要求“ ref =” reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> yme1 ，href =” http ：//www.yeastgenome.org/cgi-bin/locus.fpl？dbid = S000000549“ data-ga-action =” click_feat_suppl“ ref =” reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article ％7CFront％20Matter＆TO =外部％7CLink％7CURI“ target =” _ blank“> mgr1 或href =” http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000004721“ data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI” target =“ _ blank”> mgr3 突变，每个突变都会降低膜间空间中的i-AAA蛋白酶活性。因此，href =“ http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000002639” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Cox20 似乎也可以稳定未组装的href =” http://www.yeastgenome.org/ cgi-bin / locus.fpl？dbid = S000007281“ data-ga-action =” click_feat_suppl“ ref =” reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％ 7CURI” target =“ _ blank”> Cox2 阻止i-AAA蛋白酶降解。 <-href =“ http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000007281” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Cox2 前导肽通过href =” http://www.yeastgenome.org/cgi处理-bin / locus.fpl？dbid = S000004758“ data-ga-action =” click_feat_suppl“ ref =” reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI “ target =” _ blank“> Imp1 在没有href =” http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000002639“的情况下发生” data-ga-action = “ click_feat_suppl” ref =“ reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI” target =“ _ blank”> Cox20 和i-AAA蛋白酶活动，但效率大大降低。在这种情况下，一些成熟的href =“ http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000007281” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Cox2 被组装到细胞色素C氧化酶中，从而使呼吸道生长缓慢。因此，href =“ http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000002639” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 3276622＆issue- id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Cox20 伴侣蛋白在前导肽加工，C-tail出口和的稳定化中具有重要作用href =“ http://www.yeastgenome.org/cgi-bin/locus.fpl?dbid=S000007281” data-ga-action =“ click_feat_suppl” ref =“ reftype = extlink＆article-id = 3276622＆issue-id = 205430＆journal-id = 301＆FROM = Article％7CFront％20Matter＆TO = External％7CLink％7CURI“ target =” _ blank“> Cox2 。

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