The Lambda F Mutants Belong to Two Cistrons

摘要

Amber mutants previously mapped at seven sites in head gene F exhibit two contrasting phenotypes. Mutant F423, located in the right half of gene F, makes defective head particles with normal size λDNA. These particles are complemented by crude lysates of mutants in other head genes. Mutants F785, F471 and F730 map in three adjacent sites in the left half of gene F. They are not complemented by lysates of other head mutants and do not produce headlike particles. Their lysates complement the incomplete heads present in F423. In vivo all three mutants are complemented by F423 but not by each other.