Examination of signalling pathways involved in muscarinic responses in bovine ciliary muscle using YM-254890 an inhibitor of the Gq/11 protein

摘要

Background and purpose:In the ciliary muscle, the tonic component of the contraction produced by cholinergic agonists is highly dependent on Ca²⁺ provided by influx through non-selective cation channels (NSCCs) opened by stimulation of M3 muscarinic receptors. We examined effects of YM-254890 (YM), a Gq/11-specific inhibitor, on contraction, NSCC currents and [Ca²⁺]i elevation induced by carbachol (CCh).