Crystallization and preliminary X-ray characterization of queD from Bacillus subtilis an enzyme involved in queuosine biosynthesis

摘要

QueD (previously named ykvK) is one of several enzymes involved in the biosynthesis of the hypermodified nucleoside queuosine. Queuosine is incorporated into tRNA at position 34 of four tRNAs: tRNA^His, tRNA^Asp, tRNA^Asn and tRNA^Tyr. The crystallization and preliminary X-ray crystallo­graphic studies of queD are described here. The recombinant protein from Bacillus subtilis was overproduced in Escherichia coli and crystallized using the hanging-drop vapor-diffusion method from 25% PEG 600, 100 mM NaCl and sodium citrate buffer pH 5.5 at 291 K. The crystals diffract to 3.6 Å resolution and belong to the cubic space group F4132, with unit-cell parameter a = 240.88 Å.