参附注射液对脓毒症模型大鼠心脏血流动力学的影响

摘要

Objective: To observe the effects of Shenfu Injection(SF) on cardiac hemodynamics in rats with sepsis. Methods: A total of 80 Sprague-Dawley(SD) rats were subjected to sepsis by cecal ligation and puncture(CLP) and then were randomly divided into 4 groups: sham operation group(n=20), sepsis group(n=20), Low-SF group(n=20) and High-SF group(n=20). Mean arterial pressure (MAP), heart rate (HR), heart systolic functional parameters (LVDP and +dP/dt), heart diastolic functional parameters (LVEDP and -dP/dt) of each group were recorded in 6 h and 18 h after the surgery and then compared. Results: Compared with the sham group, HR in sepsis group increased significantly in 6h after surgery (P<0.05), but significantly decreased in 18 h after surgery(P<0.01); and MAP, LVDP, +dP/dt, LVEDP, and -dP/dt in sepsis group all dramatically decreased in 6h and 18 h after surgery (all P<0.01). Compared with sepsis group, Low-SF group had similar MAP and HR in 6h after surgery, but ele⁃vated MAP and HR in 18h after surgery(all P>0.05); High-SF group had lower HR in 6h after surgery (P<0.05) and increased HR in 18h and increased MAP in 6h and 18h after surgery(all P<0.01);and both SF groups had increased LVDP, +dP/dt, LVEDP, and -dP/dt in 6h and 18h after surgery(P<0.05 or 0.01). Conclusion: SF can increase MAP to different extent in septic rats. In addition, SF can improve cardiac systolic and diastolic func⁃tion. These results were more obvious in High-SF group. These effects may be proportional to the dose of Shen⁃fu Injection.%　　目的：观察参附注射液对脓毒症大鼠心脏血流动力学的影响.方法：采用肓肠结扎穿孔法制备腹腔感染脓毒症动物模型.80只SD大鼠随机分为假手术组、脓毒症组、参附注射液低、高剂量组，每组20只.各实验组术后给予相应处理措施，观察术后6、18h各组的平均动脉压(MAP)、心率(HR)、心脏收缩功能指标(LVDP和+dP/dt)、心脏舒张功能指标(LVEDP和-dP/dt).结果：与假手术组比较，脓毒症组术后6h HR显著升高(P<0.05)，术后18h HR显著降低(P<0.01)；术后6h和18h MAP、LVDP、+dP/dt、LVEDP、-dP/dt均显著下降(P均<0.01).与脓毒症组比较，参附注射液低剂量组术后6h MAP、HR差异无统计学意义(P均>0.05)，术后18h MAP和HR显著升高(P<0.05)；参附注射液高剂量组术后6h HR显著降低(P<0.05)，术后18h HR和术后6、18h MAP均显著升高(P 均<0.01)，参附注射液低、高剂量组术后6、18h 的 LVDP、+dP/dt、LVEDP、-dP/dt 均显著升高(P<0.05或<0.01).结论：参附注射液可升高脓毒症大鼠的平均动脉压，改善其心脏收缩和舒张功能；高剂量参附注射液作用更明显，并能改善心率.参附注射液能改善脓毒症大鼠心脏血流动力学的作用强度与剂量有关.