电针对缺血性脑卒中再灌注损害大鼠 CREB 表达的影响

摘要

目的：探寻电针对缺血性脑卒中再灌注损害大鼠cAMP反应原件结合蛋白（ cAMP-response Element Binding Protein ， CREB）表达的影响。方法：将60只健康成年的SD大鼠驯养3 d随机分成假手术组、模型组以及电针组，每组各20只，使用线栓法大脑中动脉闭塞（ Middle Cerebral Artery Occlusion ，MCAO）对模型组、电针组大鼠进行脑缺血模型制作，缺血2 h后进行再灌注，电针组大鼠接受电针干预，再灌注3 d后将大鼠断头取脑，进行2，3，5-氯化三苯基四氮唑（2，3，5-triphenyltetrazolium chloride，TTC）染色以及图像软件分析观察脑梗死体积，免疫蛋白印迹法（Western blotting）检测CREB蛋白水平，聚合酶链反应（ Reverse Transcription-polymerase Chain Reaction ，PCR）检测CREB的基因表达水平。结果：TTC及其图像软件分析表明，电针组的SD大鼠脑梗死面积明显小于模型组（ P＜0.01）；电针组的SD大鼠CREB蛋白水平明显高于模型组（ P＜0.01）；电针组的SD大鼠CREB基因水平明显高于模型组（ P＜0.01）。结论：电针改善脑缺血再灌注损伤可以通过上调CREB实现。%Objective:To explore the impact of electro-acupuncture on the expression of CREB in the ischemia-reperfusion rats .Meth-ods:Sixty healthy adult SD rats which were domesticated for 3 days were randomly divided into the shame group , the model group and the electro-acupuncture group .There were 20 rats in each group .The rats of the model group and the electro-acupuncture group were given the line bolt method ( MCAO) to complete the brain ischemia model , and were reperfused 2 hours after ischemia .The electro-acu-puncture group adopted the intervention of electro-acupuncture .Three days after the reperfusion , all the rats were beheaded and taken brains.The cerebral infarction volume was observed through TTC staining and image analysis , and the protein expression of CREB were detected by protein imprinting method ( Western blotting ) and gene expression of CREB were tested by Real-Time PCR.Results:TTC and the image analysis showed that the cerebral infarction volume of electro -acupuncture group was significantly smaller than that of the model group (P<0.01);the CREB protein level in the electro-acupuncture group was obviously higher than that of the model group (P<0.01);the CREB gene level in the electro-acupuncture group was much higher than that of the model group (P<0.01).Conclu-sion:Electro-acupuncture can realize the improvement of the cerebral ischemia reperfusion injury by up -regulating CREB.