肾康灵调控系膜细胞炎性反应因子的机制研究

摘要

Objective:To clarify the mechanism of Shenkangling on mesangial cells and to observe oxidized low density lipoprotein (ox-LDL)induced Mesangial Cells(MCs)secretion of inflammatory cytokine.Methods:MCs were treated with Shenkangling,and the levels of the inflammatory cytokines,such as CXCL1 6,CD36,IFN-γ,IL6,TNF-αby ELISA,Real Time-PCR and Western blot were detected.Results:The levels of CXCL1 6,CD36,IFN-γ,IL6,TNF-αwere significantly increased after CXCR6 joined and ox-LDL induced mesangial cell proliferation,then all levels were significantly reduced by Shenkangling in a concentration dependent manner.Conclusion:ox-LDL may promote the release of inflammatory mediators such as in mesangial cells,and CXCR6 may medi-ate this path.Shenkangling has effects for ameliorating ox-LDL-induced MC proliferation and inflammatory reactions.%目的：观察氧化低密度脂蛋白（Oxidized Low-Density Lipoprotein，ox-LDL）对系膜细胞（Mesangial Cells，MCs）分泌炎性反应递质功能的影响，并从细胞分子生物学水平阐明肾康灵的作用机制。方法：采用肾康灵干预增殖的系膜细胞，并利用分子生物学技术检测CXCL16、CD36、IFN-γ、IL6、TNF-α含量或基因水平。结果：利用ox-LDL诱导大鼠系膜细胞增殖并加入CXCR6受体后，CXCL16、CD36、IFN-γ、IL6、TNF-α的含量或基因水平显著升高，肾康灵呈浓度依赖性降低其表达水平。结论：ox-LDL诱导系膜细胞增殖时通过CXCR6介导，可促进CXCL16、CD36、IFN-γ、IL6、TNF-α等炎性反应递质的释放，中药肾康灵可通过抑制炎性反应递质的释放，保护系膜细胞的功能。