冬虫夏草水提液和醇提液HPLC指纹图谱分析

摘要

Objective:To establish chemical fingerprints of the aqueous extract and methanol extract of Cordyceps by HPLC.Methods:The aqueous extract and methanol extract of 20 batches Cordyceps and 2 batches Cordyceps mycelium were analyzed by HPLC.The chromatographic data was evaluated by similarity analysis and cluster analysis.Results:The aqueous extract and meth-anol extract chemical fingerprints of Cordyceps were developed.Seven common peaks were identified in aqueous extract and eight common peaks were identified in methanol extract.The similarities of 20 batches Cordyceps aqueous extract and methanol extract were higher than 0.9,and the similarities of 2 batches Cordyceps mycelium were lower than 0.9.The cluster analysis result showed that Cordyceps and Cordyceps mycelium were separated in different clusters.Conclusion:The developed HPLC fingerprint method is stable and reliable,which helps to the comprehensive quality evaluation of Cordyceps.%目的:建立冬虫夏草水提液和醇提液的高效液相指纹图谱.方法:采用高效液相色谱对20批冬虫夏草和2批发酵冬虫夏草菌粉的水提液和醇提液进行分析;并对所获得的色谱数据进行相似度计算和聚类分析.结果:建立了冬虫夏草水提液和醇提液指纹图谱,水提液指纹图谱确定了7个共有峰,醇提液指纹图谱确定了8个共有峰;冬虫夏草水提液和醇提液指纹图谱相似度评价结果均显示20批冬虫夏草样本的相似度系数大于0.9,2批发酵冬虫夏草菌粉的相似度系数小于0.9;冬虫夏草水提液和醇提液共有峰聚类分析结果均显示,20批冬虫夏草与2批发酵冬虫夏草菌粉分别聚为不同类.结论:本文所建立的方法稳定可靠,可以对冬虫夏草质量进行综合评价,为其提高产品质量控制提供了依据.