基于Wnt通路加味乌梅丸对结肠癌的抑制机制

摘要

目的:探讨加味乌梅丸抑制结肠癌的效果并分析其作用机制.方法:选取BALB/c雄性小鼠30只,随机分为空白组、模型组和乌梅丸组,每组10只.其中空白组小鼠不接受干预措施,乌梅丸组进行乌梅丸煎剂灌胃处理,空白组及模型组接受等剂量生理盐水灌胃,均干预28 d,观察肿瘤体积的变化、小鼠的体重及存活率、肿瘤组织Wnt、JNK水平变化.结果:模型组与乌梅丸组小鼠肿瘤体积较干预前均有增加的趋势,其中乌梅丸组增加的速度较慢,并在干预28 d后肿瘤组织有减小的趋势;干预28 d空白组小鼠体重与初始体重相近,模型组及乌梅丸组小鼠体重明显下降,其中模型组较乌梅丸组下降明显,差异有统计学意义(P＜0.05);干预结束后模型组仅有4只小鼠存活,而乌梅丸组有7只小鼠存活,存活率差异有统计学意义(P＜0.05).模型组及乌梅丸组小鼠肿瘤组织Wnt、JNK蛋白及mRNA均过表达,其中模型组Wnt、JNK表达水平明显高于乌梅丸组,差异有统计学意义(P＜0.05).结论:加味乌梅丸具有明显抑制结肠腺瘤恶化作用,其抗肿瘤机制可能与抑制Wnt通路表达有关.%Objective:To investigate the colon cancer inhibition of modified wumei boluses and to analyze some of the action mech -anism.Methods:A total of 30 BALB/c male mice were randomly divided into blank group , model group and wumei boluses group, with 10 in each group.The mice of blank group did not accept intervention measures , and wumei boluses group had lavage treatment of wumei boluses decoction.Blank group and model group received isodose physiological saline lavage.All the groups had 28 d intervention.The changes of tumor volume , weight of mice and survival rate , concentration changes of Wnt , JNK in the tumor tissue were observed.Results:1)the mice tumor volume increased in the model group and wumei boluses group than before the intervention, and the increase in the wumei boluses group was more slowly.The tumor tissue had a tendency to decrease after 28 d of intervention.2)After 28 days of the intervention, the mice weight in the blank group was similar to the initial weight , and the mice weight of the model group and the wumei boluses decreased obviously .The decrease of model group was more significantly than that in the wumei boluses group (P＜0.05); After the intervention, only 4 mice survived in the model group, and 7 mice survived in the wumei boluses group.The survival rate differences between the two groups were statistically significant ( P ＜0.05).3)Protein and mRNA of Wnt, JNK in mice tumor tissue of both the model group and wumei boluses group showed high ex-pression, and expression levels of Wnt , JNK in the model group was obviously higher than that of wumei boluses group ( P＜0.05).Conclusion:The modified wumei boluses has obvious inhibitory effect on deterioration of colonic adenoma , and the mecha-nism of antitumor may related to the inhibition on the expression of Wnt pathways .