醇提法提取向日葵花瓣中绿原酸及其超氧阴离子清除作用

摘要

Chlorogenic acid was obtained from helianthus annuus L petals via extraction method using al-cohol as the solvent. The extraction parameters were optimized through orthogonal experimental method af-ter a single-factor test. The results showed that a maximum chlorogenic acid yield of 1.801%was obtained under the optimum conditions, i.e. temperature of 50 ℃, ethanol volume fraction of 70%, pH value of 6, a solid/liquid ratio of 1:20 (g/mL) and extraction time for 30 min. Antioxidant activity test showed that chlorogenic acid from helianthus annuus L petals possessed better scavenging capability of ultra-oxygen anion O2-·than Vc.%以向日葵花瓣为原料，选用醇提法提取向日葵花瓣中绿原酸，在单因素实验的基础上，通过正交试验对工艺条件进行优化，结果表明，向日葵花瓣中绿原酸最佳提取条件：温度50℃，乙醇体积分数70%，pH值6，料液比1：20，回流时间30 min，此时，提取率可达1.801%。抗氧化性研究表明：向日葵花瓣中绿原酸提取液对超氧阴离子具有一定的清除作用，且效果比Vc好。