Objective To investigate the expression of receptor activator of nuclear factor KB ligand (RANKD and osteoprotegerin (OPG) in periapical cyst and periapical granuloma by comparison with the expression in the normal periodontal tissue as control, and to identify their functional mechanism in the bone destruction of periapical cyst and granuloma. Methods 20 periapical cyst tissues (cyst group), 20 periapical granuloma tissues (granuloma group), and 20 normal periodontal tissues (control group) were collected respectively. Irnmunohistochemical technology was performed to detect the expression of RANKL and OPG in above three groups. Results In cyst group, granuloma group and control group, the expression of RANKL were 75.00±7.54, 68.40±6.74 and 29.40±2.46, respectively. The expression of OPG were 38.10±7.09, 47.65±13.85 and 58.60 5.88, respectively. The differences among the three groups were statisti-cally significant (P<0.05). RANKL and OPG in cysts group were negatively correlated (r=-0.56, P=0.01) and were not correlated with granuloma and control group (P>Q.Q5). Conclusion RANKL and OPG play roles in the bone absorp-tion of periapical disease. In periapical disease, abnormal expression of RANKL and OPG are detected, RANKL signi-ficantly increase, OPG decrease, bone absorption accelerate and osteolytic lesion are observed. In periapical cyst, the bone absorption is more active compared with periapical granuloma.%目的 检测破骨细胞核因子κB受体活化因子配体(RANKL)和骨保护素(OPG)在根尖周囊肿及根尖周肉芽肿中的表达水平,探讨RANKL和OPG在不同根尖周疾病周围骨质破坏机制中的作用.方法 收集根尖周囊肿组织20例为囊肿组,根尖周肉芽肿组织20例为肉芽肿组,正常牙的牙周膜组织20例为对照组.采用免疫组织化学法检测RANKL和OPG在根尖周囊肿、根尖周肉芽肿和正常牙周膜组织中的阳性表达.结果 在囊肿组、肉芽肿组、对照组中,RANKL的表达分别为75.00±7.54、68.40±6.74和29.40±2.46,OPG的表达分别为38.10±7.09、47.65±13.85和58.60±+5.88,3组间的差异均有统计学意义(P＜0.01).相关性分析表明,囊肿组中RANKL与OPG呈负相关关系(r=-0.56,P=0.01),肉芽肿组和对照组中RANKL和OPG无相关关系(P＞0.05).结论 RANKL与OPG参与了根尖周病变引起的骨吸收过程.在根尖周病变中,RANKL和OPG的表达失调,RANKL增加而OPG减少,骨吸收活跃,导致溶骨性病变.根尖周囊肿的骨吸收活动较根尖周肉芽肿更活跃.
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