High-throughput 454 pyrosequencing was used to investigate the differences of microbial structures of anode biofilm in the air-cathode microbial fuel cells with different separators,i.e.,a proton exchange membrane of Nafion 117 and a polyether sulfone (PES) microfiltration membrane.AKYG597_norank belonging to Desulfuromonadales and Geobacter were the most abundant genera in these two reactors,accounting for 77.4％ and 36.4％ of the total population,respectively.The oxygen diffusion rate was elevated via the utilization of PES membrane as separation,which increased the relative abundances of facultative anaerobe such as Rhizobium,Xanthobacter and Acinetobacter.Due to the relatively low resistance of PES membrane,the air-cathode MFCs with separator of PES membrane achieved higher energy output.%利用高通量454焦磷酸测序手段研究了分别以Nafion 117质子交换膜与聚醚砜(PES)微滤膜为分隔物的空气阴极MFCs长期运行时阳极表面生物膜的微生物群落结构差异.结果显示,两套反应器阳极上相对丰度最高的细菌分别为属于除硫单胞菌目(Desulfuromonadales)的AKYG597_norank与地杆菌属(Geobacter),各占到总体的77.4％与36.4％.利用PES微滤膜作为分隔物的MFCs氧扩散率增加,因此阳极生物膜中根瘤菌(Rhizobium)、黄色杆菌(Xanthobacter)及不动杆菌(Acinetobacter)等需氧、兼性厌氧细菌丰度得到了提升;同时,由于PES微滤膜的电阻率更低,使MFC获得了更高的能量产出.
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