电阻抗式传感器大肠杆菌快速检测方法研究

摘要

The front-end design of the impedance-based biosensor for rapid detection of bacterial has been completed based on the impedance microbiology. Proper experimental scheme is designed to research the impedance change during the Escherichia coli growth. The slope of Nyquist plots is chosen to characterize the bacterial quantity. The measurement results is analyzed,and experiments are designed to verify it. The changes are explained in the Nyquist slope during the bacterial growth. And the results are in agreement with the theoretical derivation. The analysis method reduces the requirements of microelectrodes and measuremental environment and shortens the time, which can be used for rapid detection of microorganisms.%基于电阻抗法原理,完成了细菌快速检测阻抗式传感器的前端设计,并对叉指式电极进行工艺实现；合理设计实验方案,选取奈奎斯特曲线斜率表征细菌数量的变化,完成了大肠杆菌生长过程的阻抗研究；对测量结果进行分析,并设计实验验证,完成了细菌生长过程中奈奎斯特斜率变化的理论分析,其结果与理论推导吻合.该数据分析方法降低了对测试电极和测量环境的要求,缩短了细菌测定所需要的时间,可用于微生物的快速检测中.