Analysis of the action mechanism of Fang Ji Huang Qi decoction in treating rheumatoid arthritis by network pharmacology

摘要

目的:通过网络药理学探讨防己黄芪汤治疗类风湿性关节炎的药理学机制.方法:通过中药系统药理学分析平台(TCMSP)检索中药的化学成分和作用靶点,并于UniProtKB网络平台获取每种靶蛋白的基因名.通过CTD数据库查询类风湿性关节炎的疾病靶点.在STRING数据库中构建蛋白-蛋白相互作用网络(PPI),在Cytoscape中进行网络可视化分析.通过DAVID数据平台对关键靶蛋白进行基因本体论(GO)分析,京都基因与基因组百科全书(KEGG)富集通路分析.结果:从TCMSP数据库中共筛选得到472个药物有效成分,从CTD数据库中筛选出75个疾病靶点.化合物-靶点网络图中包含98个进一步筛选出的药物有效成分和相应的75个靶点,关键化合物有槲皮素、山柰酚等,关键靶点为PTGS2和NOS2等.PPI网络包括75个蛋白,关键蛋白为37个,涉及TP53,JUN,IL6等.GO条目260个,生物功能条目最多,共246个.KEGG通路共55条,主要涉及肿瘤信号通路,Toll样受体信号通路,NOD样受体信号通路.结论:本研究结果初步验证了防己黄芪汤治疗类风湿性关节炎的基本药理作用及其机制,并为进一步深入揭示其作用机制奠定了良好基础.%Objective:To explore the pharmacological action mechanism of Fang Ji Huang Qi decoction (FHD) in the treatment of rheumatoid arthritis (RA) by network pharmacology. Methods:The chemical compositions and functional targets of the TCM were retrieved using the systematic pharmacological analysis platform TCMSP, and the gene name of each target protein was obtained from the UniProtKB network platform. The targets of RA were queried through the CTD database. The protein–protein interaction network was constructed in the STRING database, and the network visualization analysis was performed in Cytoscape. The Gene Ontology and Kyoto Gene and Genomic Encyclopedia pathways enrichment analyses of key target proteins were performed using the DAVID data platform. Results: A total of 472 drug active ingredients were screened from the TCMSP database. Seventy-five disease targets from the CTD database were screened. The compound-target network map contained further screened out 98 components and corresponding 75 targets. The key compounds included quercetin and kaempferol. The key targets were prostaglandin G/H synthase 2 and nitric oxide synthase 2. The protein-protein interaction network consisted of 75 proteins, of which 37 were key proteins, including tumor protein 53, JUN and interleukin-6. There were 260 Gene Ontology entries, of which 246 were biological processes. Fifty-five Kyoto Gene and Genomic Encyclopedia pathways were enriched, mainly the cancer pathway, NOD-like receptor signaling pathway, and Toll-like receptor signaling pathway, which are involved in the action mechanism of FHD. Conclusion:The results of this study preliminarily verified the basic pharmacological action mechanism of FHD in the treatment of RA, laying a foundation for elucidating its mechanism of action.