Pseudolaric acid B extracted from the Chinese medicinal herb Cortex Pseudolaricis ameliorates DNFB-induced atopic dermatitis-like skin lesions in BALB/c mice

摘要

Objective:Pseudolaric acid B (PB) is a newly identified diterpenoid isolated from Tujinpi (Cortex Pseudolaricis). In the present study, we aimed to explore the anti-inflammatory effects of PB on atopic dermatitis (AD), as well as the molecular mechanisms underlying its effects. Methods:BALB/c mice treated with 2,4-dinitrofluorobenzene were orally administered with PB (10 mg?kg-1?d-1). After evaluating the AD score, serum levels of IgE and the mRNA expression of NLRP3 inflammasome and IL-1βwere measured by ELISA and qRT-PCR respectively. Results:The results showed that PB treatment significantly ameliorated the development of AD-like clinical symptoms and effectively suppressed the infiltration of inflammatory cells. Furthermore, PB inhibited the expression of NLRP3 inflammasome and IL-1βin skin lesions, and downregulated serum IgE levels. Conclusion: The anti-inflammatory properties of PB were demonstrated using the 2,4-dinitrofluorobenzene-induced mouse model of AD-like skin lesions. Our study highlighted the potential use of PB as a novel therapeutic agent for the treatment of inflammation-associated skin diseases.%目的:探讨取自土槿皮中的二萜酸土槿乙酸对特应性皮炎的抗炎免疫调节作用,并分析其作用机制.方法:以二硝基氟苯刺激诱导BALB/c小鼠建立特应性皮炎动物模型,同时每日给予口服土槿乙酸干预(10 mg/kg/d),计算SCORAD评分,分别采用ELISA和qRT-PCR的方法检测血清IgE含量以及NLRP3炎症小体的基因表达.结果:土槿乙酸能明显减轻特应性皮炎模型小鼠的临床症状,抑制皮损处炎症细胞浸润.此外,土槿乙酸还能显著下调皮损组织中NLRP3和IL-1βmRNA水平及血清IgE含量.结论:土槿乙酸通过抗炎免疫调节作用抑制二硝基氟苯诱导的特应性皮炎,提示土槿乙酸可能成为治疗炎症相关疾病的新型候选化合物.