不同产地和部位对片烟自然醇化过程中细菌群落结构的影响

摘要

To investigate the differences of bacterial community structure among tobacco strips from different growing areas and stalk positions during aging, the bacterial 16S rDNA of strip samples of upper, middle and lower leaves from 4 areas (Youxi in Fujian, Zhanyi in Yunnan, Changshun in Guizhou and Queshan in Henan) during aging were sequenced by Illumina MiSeq. The results showed that 940 operational taxonomic units (OTUs) were obtained from 12 samples. The samples (C3F) from Youxi had the most abundant bacterial biodiversity. The dominant phyla in 12 samples were Proteobacteria, and the dominant genera in most samples were Sphingomonas. The Venn diagram of bacterial OTUs of strips from different areas during aging revealed that 195 of OTUs were common to the strips from 4 areas, the numbers of specific OTUs for Youxi, Zhanyi, Changshun and Queshan were 300, 9, 4 and 0, respectively.%为探明不同产地及不同部位的片烟在自然醇化过程中细菌群落结构的差异,以福建三明尤溪、云南曲靖沾益、贵州黔南长顺、河南驻马店确山4个产地,上中下3个烟叶部位的12份自然醇化片烟为材料,利用Illumina MiSeq测序平台对自然醇化片烟中细菌16S rDNA进行高通量测序分析.结果表明:12份自然醇化片烟样品共获得940个操作分类单元(Operational taxonomic unit,OTU).福建三明尤溪C3F自然醇化片烟细菌群落的多样性最丰富.12份样品细菌群落的优势菌门均为变形菌门,大多数样品的优势菌属为鞘氨醇单胞菌属.不同产地自然醇化片烟细菌OTU的韦恩图分析表明,4个产地共有的OTU数量为195个,福建三明尤溪、云南曲靖沾益、贵州黔南长顺和河南驻马店确山特有的OTU数量分别为300、9、4和0个.