血小板微颗粒对人脐静脉内皮细胞黏附因子表达的影响

摘要

目的:探讨血小板微颗粒(PMPs)对人脐静脉内皮细胞黏附因子表达的影响及PMPs在冠心病中的可能作用机制.方法:分离制备血小板微颗粒,与人脐静脉内皮细胞CRL-1730共培养,应用半定量逆转录聚合酶链反应技术测定不同浓度PMPs作用不同时间的内皮细胞黏附因子E-选择素、细胞间黏附分子(ICAM)-1、血管细胞间黏附分子(VCAM)-1的表达.结果:当PMPs达到一定浓度,可促使内皮细胞(CRL-1730)表达E-选择素、ICAM-1和VCAM-1升高,差异有统计学意义(P＜0.05);而PMPs刺激内皮细胞(CRL-1730)不同时间,E-选择素、ICAM-1的表达差异无统计学意义(均P＞0.05).结论:PMPs可促使体外培养的人脐静脉内皮细胞(CRL-1730)表达E-选择素、ICAM-1和VCAM-1升高,从一方面解释了PMPs在冠心病中的可能的作用机制.%Objective: To investigate the effects of platelet microparticles (PMPs) on the expressions of cell adhesion molecule in human umbilical vein endothelial cells ( HUVECs), and the merhanisms of' PMPS in coronary heart disease thereof: Methods: HUVECs (CRL-1730) and prepared PMPs were co-cultured. Reverse transcription-polymerase chain reaction (SQRT-PCR) was used to detect the relative expressions of E-selectin, CAM-1 and VCAM-1 in endothelial cells respectively.Results: The expression levels of E-selectin. ICAM-1 and VCAM-1 were significantly increased when HUVECs(CRL-1730) were interfered by PMPs ( P ＜ 0.05). But no significant differeces were found in the expressions of E-selectin, ICAM-1and VCAM- 1 when HUVECs (CRL-1730) were stimulated hy PMPs at different times (P ＞ 0.05). Conclusion: The PMPs may increase the expressions of E-selectin. ICAM-1 and VCAM-1 of HUVECs(CRL-1730), which may explain a possible mechanism of PMPs in coronary heart disease.