酶解除去蔗汁中的右旋糖酐

摘要

右旋糖酐对制糖过程以及糖产品的危害早已在国内外引起高度关注。目前除去蔗汁中存在的右旋糖酐最有效的方法是酶解。在单因素实验基础上，通过响应面法优化，建立回归模型，得到应用右旋糖酐酶催化水解蔗汁中右旋糖酐的最佳反应条件：酶剂量0.05 U/mL蔗汁、pH5.3、反应时间15 min、反应温度51℃。在该条件下，可将蔗汁中含量为800～900 mg/kg°Bx的右旋糖酐完全去除。%The hazard of dextran in sugar manufacture has been highly concerned around the world. The application of dextranase is the most effective method to breakdown dextran. The optimization of dextranase to hydrolyze dextran in sugarcane juice was carried out by using response surface methodology. A Box-Behnken factorial designed with four factors and three levels, was used to determine the influence of enzyme dosage (U/mL), pH, temperature (℃) and reaction time (min), and regression model was built. The optimum operational conditions were as follows:enzyme dosage 0.05 U/mL, pH value 5.3, reaction time 15 min and temperature 51℃. In this condition, dextranase can hydrolyze all of 800~900 mg/(kg·˚Bx) dextran in sugarcane juice.