A novel molecular hyperspectral imaging (MHSI) system based on AOTF (acousto-optic tunable filters) was presented. The system consists of microscope, AOTF-based spectrometer, matrix CCD, image collection card and computer. The spectral range of the MHSI is from 550 to 1 000 nm. The spectral resolution is less than 2 nm, and the spatial resolution is about 0. 3 fan. This paper has also presented that spectral curves extracted from the corrected hyperspectral data of the sample, which have been preprocessed by the gray correction coefficient, can more truly represent biochemical characteristic of the sample. The system can supply not only single band images in the visible range, but also spectrum curve of random pixel of sample image. This system can be widely used in various fields of biomedicine, clinical medicine, material science and microelectronics.%设计出一种基于AOTF(acousto-optic tunable filters)的分子超光谱成像系统(MHSI).整个系统由显微镜、分光计、CCD镜头、数据采集卡和计算机等几部分组成.系统的光谱范围为550～1 000 nm,可采集225个波段,光谱分辨率优于2nm,空间分辨率达到0.3μm.由于系统的光源对样本的光谱有较大影响,本文提出了灰度校正系数算法对数据进行相应的预处理,并对系统进行辐射校正,实验结果表明经过预处理后消除了光源的影响,能更真实的反映样本的生化特征.本系统不仅能提供物体在可见光范围的单波段图像,而且能获得图像中任意像素的光谱曲线,实现了光谱技术与成像技术的结合,可广泛应用于生物医学,临床医学,材料学,微电子学等学科领域.
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