激光拉曼光谱法无损鉴别人参及其伪品

摘要

The objective of the present study is to identify Ginseng and its false sample: Anthriscus sylvestris, Radix glehniae and balloonflower root by Laser Raman spectroscopy, second derivative Raman spectroscopy. The structural information of the samples indicated that Ginseng and its false samples contains a large amount of carbohydrates, since some characteristic vibration peaks of the carbohydrates, such 1 640, 1 432, 1 130, 1 086, 942, 483 cm-1 can be observed. The characteristic vibration peak of Radix glehniae which arouse at 2 206 cm-1 in the aman spectra, totally different from the other three kinds of traditional Chinese medicines. Anthriscus sylvestris appeare the characteristic vibration peak in 1 050 cm-1 and the corresponding 1 869 cm-1 because of the chain ester compounds. The characteristic vibration peak of balloonflower root, such as 1 227, 691, 600 cm-1 can be observed, significantly different from the other three herbs in the Raman spectra. Further more, the previous identification results can be verified again with second derivative Raman spectroscopy. This identification method is more fast, convenient, and keeping the integrity of the samples than the routine spectroscopic method.%利用激光拉曼光谱技术并结合二阶导数拉曼光谱,对人参及其拟伪品峨参、北沙参、桔梗进行了鉴别.人参及其伪品均在拉曼光谱中出现了1 460,1 130,1 086,942,483 cm-1等拉曼振动峰,根据这些拉曼位移可以判断出在人参及其伪品中都含有糖类物质.北沙参的拉曼谱图中出现了不同于其他三种药材的2 206 cm-1的拉曼特征峰.峨参中出现了1 050和1 869 cm-1相对应的链状酯类化合物的拉曼振动峰.桔梗中出现了1 227,600,691 cm-1等明显不同于其他三种药材的拉曼振动峰.利用这些拉曼振动峰的差异可将人参及其伪品进行很好的区分.再利用二阶导数拉曼光谱图对人参及其伪品的鉴别结果进行进一步的补充说明.此鉴别方法与常规的光谱法相比具有更直接、快速,并且具有不破坏样品的原性质的特点.