表面增强拉曼光谱（SERS）技术对非标记蛋白质的研究进展

摘要

This article outlines the recent progress in surface enhanced Raman spectroscopy (SERS)based proteins applications. SERS is a specific Raman spectroscopic technique that provides enhanced Raman signals (several orders of magnitude greater than normal)for numerous Raman-active analyte molecules adsorbed onto rough metal surfaces.SERS is a sensitive,selective, and versatile technique that lends to fast data acquisition in actual time.Therefore,SERS has undergone rapid development be-cause of its technical advantages in instrumentation,data analysis methods and its multiple biological applications.This article highlights several representative areas in proteins where SERS could be employed.Some of the proteins applications of SERS are more maturely developed,whereas others are in their initial stages of development (in laboratories).This article discusses the recent developments in SERS based quantitative analysis:directly with different substrates (e.g.biomolecules on electrodes, colloidal particles,and periodic pattern structure and tip based substrates).Furthermore,SERS based techniques are advanta-geous for obtaining valuable information on protein-protein,protein-ligand,and protein-drug recognitions via spectral differences among molecular bridges.SERS based techniques show considerable promise for qualitative and/or quantitative analyses of bio-logical systems.%基于表面增强拉曼光谱(SERS)技术在非标记蛋白质研究方面的最新进展。SERS是一个特殊的拉曼光谱现象,对于众多被吸附到粗糙金属表面上的拉曼活性分析物,可以提供增强拉曼信号(通常可以增强几个数量级)。SERS是一个灵敏的,选择性的,和通用的技术,并且可以实时、快速的对数据进行采集。因此,在基于仪器仪表技术和数据分析方法以及 SERS在生物体系中的诸多优势,SERS经历了快速的发展阶段。重点介绍几个采用 SERS技术对生物体系的代表性研究。某些 SERS的生物应用发展比较成熟,并已经可以小范围临床应用,而有些还停留在发展的初始阶段(实验室研究阶段)。讨论了最近发展起来的几种基于 SERS技术定量分析的方法,选择不同 SERS活性基底和技术(如生物分子在电极上,胶体纳米粒子,周期性图案结构和基于针尖拉曼技术)对蛋白质进行直接研究。此外,根据 SERS指纹信息的变化可以用来研究蛋白质-蛋白质,蛋白质-配体间的相互作用。基于 SERS 技术对生物分子进行定性和/或定量分析方面显示出了相当大的优势。