活血化瘀汤药队的表面增强拉曼散射光谱研究

摘要

在中药治疗过程中,以方剂为载体,注重整体,采用辨证论治的方法. 为揭示方剂的药队规律,根据药队协同效应设计法,将活血化瘀汤方剂拆分为"调和"、"化瘀生新"、"活血止痛"、"补气"四个药队方剂. 由于银纳米粒子可与煎剂产生强交互作用,并极大地增强拉曼信号,获取活血化瘀汤、调和药队煎剂、化瘀生新药队煎剂、活血止痛药队煎剂和补气药队煎剂五种方剂煎剂的表面增强拉曼散射(SERS)光谱,并对所有SER S谱峰进行归属. 主要针对各煎剂中的18个拉曼信号(523,538,622,647,732,959,977,1 003,1 048,1 077,1 145,1 245,1 326,1 402,1 456,1 470,1 518和1 605 cm-1)进行讨论. 活血化瘀汤SERS光谱中保留了药队煎剂的某些拉曼峰,如: 538,622,647,732,959,1 003,1 048,1 326,1 402,1 456,1 470,1 518和1 605 cm-1. 药队煎剂SERS光谱的某些拉曼峰并未在活血化瘀汤中出现,如: 1 077,1 145和1 245 cm-1. 在活血化瘀汤SERS光谱中,出现了新的拉曼峰,如: 523和977 cm-1. 新的拉曼峰出现说明新物质的产生. 实验结果表明,活血化瘀汤所包含的药物成分并非是各药队煎剂所含药物成分的简单相加,SE RS光谱可为方剂药队规律研究提供一优异、有效和准确的检测方法.%In integrated treatment of traditional Chinese m edicine,prescriptions are used as carriers.Attention has been given to the int egration,and the methodology of treatment is based on syndrome differentiation.To reveal the rule of drug group in prescription,according to the own drug efficacy of traditional Chinese medicine,Huo-Xue-Hua-Yu decoction (HXHYD) w as divided into four drug-group decoctions as follow:Tiao-He drug group decoc tion (THDGD),Hua-Yu-Sheng-Xin drug group decoction (HYSXDGD),Huo-Xue-Zhi-Tong drug group decoction (HXZTDGD) and Bu-Qi drug group decoction (BQDGD).T here are strong interactions between Ag nanoparticles and decoction,thus leadin g to a tremendous enhancement in the intensity of the decoction Raman scattering,the Surface-enhanced Raman Scattering (SERS) spectroscopy of HXHYD,THDGD,HY SXDGD,HXZTDGD and BQDGD were obtained and analyzed,and the characteristic Rama n bands of five decoctions were tentatively assigned.Mainly eighteen Raman sign als (523,538,622,647,732,959,977,1 003,1 048,1 077,1 145,1 245,1 326,1 402,1 456,1 470,1 518 and 1 605 cm-1) i n five decoctions were discussed.Some Raman bands in SERS spectra of drug group decoctions were retained in the HXHYD,such as 538,622,647,732,959,1 003,1 048,1 326,1 402,1 456,1 470,1 518 and 1 605 cm-1.Ho weve r so me Raman bands never appeared in the HXHYD,such as 1 077,1 145 and 1 245 cm-1.New Raman bands were generated in the SERS spectra of HXHYD,such as523 and 977 cm-1,which meant new created chemical compositions.The re sults showed that medical ingredients in the HXHYD were not the simple addition of THDGD,HYSXDGD,HXZTDGD and BQDGD,and SERS technique has great potential for providing a novel method for effectively and accurately studying drug group rule of prescription.