6种中草药对4种淡水鱼致病菌体外抑菌作用的研究

摘要

In this study, six Chinese herb medicines were tested for their antibacterial activity against 4 pathogens of freshwater fish:Aeromonas sobria, A.hydrophila, Pseudomonas fluorescent biotype Ⅰ and Flavobacterium columnaris.Using the aqueous extracts of 6Chinese herbal medicines, Coptis chinensis, Rhem officinale, Forsythia suspensa, Houttuynia cordata, Magnolia officinalis and Folium isatidis, we measured the diameter of the zone of inhibition by a filter paper disc test and determined the minimal inhibitory concentration by concentration gradient method.Results indicate that the aqueous extract of C.chinensis has the strongest inhibitory effect on the 4 pathogens, then come R.officinale and F.suspensa, while H.cordata, M.officirulis and F.isatidis do not have significant inhibitory effects.%研究了6种中草药对4种淡水鱼致病菌温和气单胞菌(Aeromonas sobria)、嗜水气单胞菌(A.hydrophila)、生物Ⅰ-荧光假单胞菌(Pseudomonas fluorescent biotype Ⅰ)和柱状黄杆菌(Flavobacterium columnaris)的体外抑菌作用.以黄连(Coptis chinensis)、大黄(Rhem officinale)、连翘(Forsythia suspensa)、鱼腥草(Houttuynia corda-ta)、厚朴(Magnolia officinalis)和大青叶(Folium isatidis)6种中草药的水提物为研究对象,采用滤纸片法检测其对4种致病菌的抑制活性,并分别测定了这6种植物提取物对供试菌的最小抑菌浓度(MIC).结果表明,黄连水提物对4种淡水鱼致病菌的抑菌效果最好,大黄和连翘水提物次之,而鱼腥草、厚朴和大青叶水提物的抑菌效果不明显.