依托咪酯对急性脑缺血大鼠海马 CA1区细胞凋亡与胱天蛋白酶-3及胱天蛋白酶-10蛋白表达的影响

摘要

Objective To investigate the effect of etomidate on acute cerebral ischemia in rat hippocampal CA1 cells apoptosis and the caspase‐3 and caspase‐10 expression influence .Methods With intraluminal suture method in rats with middle cerebral artery ischemia reperfusion model (MCAO) .Application of in situ end labeling method to detect apoptosis , Western‐blotting assay for detection of caspase‐3 and caspase‐10 protein expres‐sion.Results ①Cerebral ischemia rats hippocampal area CA 1 is of 3 h appearance of apoptosis positive cells ,48 h peak ,72 h apoptosis positive cells began to decline .Etomidate group hippocampal CA1 area of rats after adminis‐tration of the number of apoptotic cells in reperfusion 6‐72 h at each time point were lower than those in ischemia group ( P<0 .05 ) .②The etomidate group in hippocampal CA1 region of rats with caspase‐3 and caspase‐10 pro‐tein expression in after the administration of 6‐72 h reperfusion time at each time point were lower than those in is‐chemia group (P<0 .05 ) .Conclusion Etomidate can inhibit acute cerebral ischemia induced cell apoptosis.%目的：探讨依托咪酯对急性脑缺血大鼠海马CA1区细胞凋亡与胱天蛋白酶（caspase）‐3及胱天蛋白酶（caspase）‐10表达的影响。方法线栓法制作大鼠大脑中动脉缺血（MCAO）再灌注模型。应用原位末端标记法检测细胞凋亡，蛋白印迹法检测caspase‐3及caspase‐10蛋白表达。结果①脑缺血组大鼠海马CA1区3 h即出现凋亡阳性细胞，48 h达到高峰，72 h凋亡阳性细胞的数量开始下降。依托咪酯组大鼠海马CA1区凋亡细胞数于给药后再灌注6～72 h各时间点均低于脑缺血组（ P ＜0．05）。②依托咪酯组大鼠海马CAl区caspase‐3及caspase‐10蛋白表达于给药后再灌注6～72 h各时间点均低于脑缺血组（ P ＜0．05）。结论依托咪酯能够抑制急性脑缺血损伤引起的细胞凋亡。