不同加工方法的棠梨叶中总多糖含量测定

摘要

We determined polysaccharides content of two manufactures from the leaves of Pyrus betulaefolia Bge. using phenol-vitriol method. With the contrast, glucose, we measured their absorbency at the wavelength of 490 nm. A good linear relationship exists between the absorbency and the determined content in the range of0. 01968 - 0. 0984 mg. Recovery experiment indicates that the average recovery rate is 99.02 % （ n = 5） and that RSD is 3.34 %. Determination results of total polysaccharide contents from different processes are 7.82% for that prepared by the traditional process of drying by sun and dewing in night and .5.27% for that prepared by sun-baked till to red after humidity accumulation. Experimental results show that the content of total polysaccharide in the leaves of P. betulaefolia Bge. is high. It can therefore be utilized as a total polysaccharide rich medical resource.%用苯酚-硫酸法测定了棠梨叶两种加工制品的多糖含量。以葡萄糖为对照品，在波长490nm处测定吸光度，在0．01968—0．0984mg范围内吸光度与被测含量具有良好的线性关系。回收实验表明平均回收率为99．02％（n=5），相对标准偏差3．34％。在此条件下，不同加工方法所得棠梨叶总多糖含量测定结果分别为：日晒夜露传统制法7．82％。渥堆变红后日晒法5．27％。实验证明：棠梨叶总多糖含量较高，可作为富含多糖的药物资源加以开发利用。