以银杏内酯(白果内酯、银杏内酯A、银杏内酯B和银杏内酯C)的提取率为指标,确定银杏叶乙醇提取的最佳提取工艺.采用正交试验设计的方法,系统考察提取时间、料液比、乙醇体积分数和提取次数4个因素对银杏叶提取工艺的影响.以异丙醇为流动相,优化高效液相色谱法(HPLC-ELSD)测定银杏内酯的含量,并计算提取率.实验得出最佳提取工艺条件为提取时间1.5 h、料液比1:20、乙醇体积分数50%、提取次数3次.本工艺可以提高银杏内酯的提取率,节约试验成本,适用于工业化生产.%We determined optimum extraction condition of Ginkgo biloba leaves with the extraction rate of ginkgolides (ginkgo lactone, ginkgolides A, ginkgolide B and ginkgolide C) as an index. We employed orthogonal experiment to systematical y investigate the impact of extraction time, solid-liquid ratio, ethanol concentration, and extraction time on extraction technology of Ginkgo biloba leaves. Ginkgolides content was determined through optimization of high performance liquid chromatography ( HPLC-ELSD) and isopropanol of mobile phase. We also calculated extraction rate. Optimum extraction conditions are extraction time of 1. 5 h, solid-liquid ratio of 1:20, ethanol concentration of 50% and extraction times of 3. This technology can improve extraction rate of ginkgolides, save testing cost, and is applicable to industrial production.
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