采用荧光定量RT-PCR法检测36例不同类型白血病患者的多药耐药基因（mdr1mRNA）表达，同时检测15例骨髓或外周血正常患者，以做对照。结果对照组均为阴性表达，初治患者组mdr1阳性基因拷贝数平均为2.7×103拷贝／μgRNA；复发难治患者组为1.0×104拷贝／μgRNA（P＜0.05）。表达阴性mdr1与mdr1阳性患者的完全缓解（CR）率分别为82％和21％（P＜0.05）。提示荧光定量RT-PCR检测mdr1基因表达结果用绝对拷贝数表示，其定量准确、可靠。临床急性白血病化疗耐药的发生主要与mdr1阳性高表达有关。%The expression of multi-drug resistance gene（mdr1 mRNA）wasmeasured in 36 patients with different type of acute leukemia and in 15 normal controls by fluorogenic probe quantitative RT-PCR（FQ-RT-PCR）.Result showed that the mdr1 mRNA was neg ative in normal controls． The average level of the mdr1 gene express in untreated group was 2.7×103 copies／μgRNA，while that of clinical resistance group was 1.0×104 copies／μgRNA（P＜0.05）.The CR rate differed significantly between mdr1 positive group（21％）and negative group（82％）（P＜0.05）.It suggests that FQ-RT-PCR is an acurate quantitative method in detecting the mdr1 gene expression，a high level of mdr1 expression correlated closely with drug resistance in clinical leukemia chemotherapy.
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