稳恒磁场照射对人鼻咽癌CNE-2细胞生长与凋亡的影响

摘要

目的 研究稳恒磁场对体外培养的人鼻咽癌细胞(CNE-2)生长抑制与凋亡作用的影响.方法 利用磁感应强度为7 mT的自制稳恒磁场对体外培养的CNE-2细胞进行照射,经1、3、9、12、24、36、48、60、72 h不同时间照射处理后,采用台盼蓝拒染法进行活细胞计数,MTT法检测细胞生长活性,HE染色观察细胞形态学改变,琼脂糖凝胶电泳观察细胞凋亡情况.结果 从照射9h起细胞生长受到显著抑制(P＜0.05),生长曲线结果与MTT实验具有良好的一致性.经72 h照射处理后,MTT法、HE染色观察细胞出现形态学改变,但琼脂糖凝胶电泳检测未见细胞DNA断裂现象.结论 7 mT稳恒磁场照射对体外培养的CNE-2细胞生长有显著的抑制作用,而对诱导细胞凋亡的效果有待进一步研究.%Objective To study the influence of steady magnetic field on the growth and apoptosis of human nasopha-ryngeal carcinoma cells CNE-2. Methods CNE-2 cells cultured in vitro were exposured by the self-made steady magnetic field with the magnetic induction intensity of 7 mT , after 1,3,9, 12, 24, 36, 48 , 60, 72 h of insolation magnetism processing , Trypan blue exclusion was used to determine the viable count , MTT was used to detect the growth rate of cells; the morphologic change of cells was observed by HE, DNA degradation was detected by the agarose gel electrophoresis. Results The remarkable depressive effect of magnetic field for the cell growth starting from 9 h (P <0. 05 ) , the growth curve of CNE-2 cells had good consistency with MTT result. After 72 h insolation magnetism processing , the cells showed morphologic changes, but had no DNA breakage. Conclusions 7 mT steady magnetic fields have significant depressive effect to CNE-2, but the effect of inducing cell apoptosis need further study.