目的 探讨123I-脱氧尿嘧啶核苷(125I-UdR)对体外培养的A549肺癌细胞的杀伤作用及其影响因素.方法 测定A549肺癌细胞在含有不同浓度125I-UdR的RPMI 1640培养液中培养48 h后细胞及细胞核摄取125I-UdR的量；测定肺癌细胞A549及脐静脉内皮细胞(ECV304)在含有100 kBq/ml 125I-UdR的RPMI 1640培养液中培养2～48 h后不同时间细胞摄取125I-UdR的量；用细胞克隆形成法评价不同浓度125I-UdR对A549肺癌细胞的杀伤作用.结果 A549细胞和细胞核摄取125I-UdR的量随培养基中125I-UdR浓度增加而增加；在含有100 kBq/ml浓度125I-UdR的培养液中,随培养时间的延长A549细胞和细胞核摄取125I-UdR的量增加.在不同培养时间下,A549细胞摄取125I-UdR的量在各时间点均明显高于ECV304细胞的摄取量.细胞存活分数随培养基中125I-UdR浓度增加呈递减趋势.结论 125I-UdR能够被A549肺癌细胞摄取,并快速进入细胞核内,且摄取量存在浓度依赖性和时间依赖性,125I-UdR对肺癌细胞具有明显的杀伤作用.%Objective To investigate the killing effect of 5-[ 125I] iodo-2'-deoxyuridine (L25i-UdR) on lung cancer cell line A549 and the factors of influencing in vitro. Methods The amount of 125 I-UdR taken by the cells and caryons were measured through counting the radioactivity after incubating for 48 hours in RPMI 1640 culturing medium containing different concentration of 125I-UdR. The amount of 123I-UdR taken by A549 and ECV304 cells were measured in 100 kBq/ml dosage of 125I-UdR after incubating different time (2-48 hours). The killing effect of L25i-UdR on A549 cell was evaluated by colony forming method. Results The amount of L25i-UdR taken by the cells and karyons increased with the increasing of 125I-UdR concentration. The concentration that A549 and ECV304 cells ingested 125I-UdR was time-dependent among 2-48 hours. The radioactivity concentration of 125I-UdR uptook in A549 cell was evidently higher than that in ECV304 cell in different incubation time. The surviving fraction was gradually decreased with the increasing of 125I-UdR concentration in culturing medium. Conclusions 125I-UdR could be ingested by A549 cell in culturing medium, which showed strong killing effect on the cells. The concentration of 125I-UdR ingestion was influenced by the dose and culturing time.
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