中国大陆汉族肾移植受者CYP3A5和MDR1基因多态性研究

摘要

Objective To estahlish the detect method of gene polymorphisms of CYP3A5 and MDR1 (3435, 2677and 1236 ) , and study their distrihutions in Hans renal transplant recipients in China.Methods We designed the primers and amplified the objective DNA by PCR at the same time, then detected the gene polymorphisms of CYP3A5 and MDR1(3435, 2677 and 1236) with DHPLC.The clinical data consisted of 274 Hans renal transplant recipients from 12 provinces in China, whose distributions of CYP3A5 and MDR1 ( 3435. 2677 and 1236 ) were analyzed by x2 test.Result The genic mutations of CYP3A5 * 3 , MDRI 3435C ＞ T, MDR1 2677G ＞ T/A and MDR1 1236C ＞ T existed in Hans renal transplant recipients in China, whose frequencies were as follows 74.1％ , 49.5％ , 39.9％/13.7％ and 72.1％ , and their distributions hetween different provinces had no statistical distinctions.Conclusion There are no differences in genic mutations of CYP3A5 and MDR1 ( 3435 , 2677 and 1236 ) in Hans renal transplant recipients in different provinces of China.but the research should be lucuhrated in Hans of pure genetic gene and minority groups.%目的 建立细胞色素P450-3A5(CYP3A5)和多药耐药基因1(MDR1)3435、2677、1236基因多态性检测方法,并研究中国大陆汉族肾移植受者其基因多态性分布情况.方法 自行设计引物,采用PCR法一次性扩增目的 DNA,变性高效液相色谱技术检测分型.对在解放军第474医院肾移植中心及南方医院器官移植中心行肾移植手术及复诊的274例来自中国大陆12个省份的汉族肾移植受者CYP3A5和MDR1 3435、2677、1236基因多态性及分布情况进行分析,并观察是否存在省份差异,统计学方法 采用多个独立样本比较的χ2检验.结果 中国大陆汉族肾移植受者存在CYP3A5*3、MDR1 3435C>T、MDR1 2677G>T/A、MDR1 1236C>T基因变异,其变异频率分别为74.1%、49.5%、39.9%/13.7%、72.1%,不同省份间无统计学差异.结论 中国大陆不同省份间汉族肾移植受者CYIYJA5*3、MDR1(3435、2677、1236)基因变异无明显差异,但对于遗传基因相对较纯的小群体汉族和少数民族而言,仍有深入研究的意义.