NF-κB信号传导通路相关基因不同表型肝癌患者尿8-OHdG水平观察

摘要

Objective To investigate the difference of urinary 8-OHdG levels among varied genotypes of NF-kB signaling pathway genetic polymorphisms in hepatocellular carcinoma patients. Methods TNF-α, NF-kB, iNOS, COX-2 phenotype of NF-kB signaling pathway in 155 cases of hepatocellular carcinoma ( HCC group) were detected by PCR-RFLP method. Urinary 8-OHdG levels in HCC group and control groups were detected by ELISA. Results Urinary 8-OHdG levels in HCC group was (9.29 ± 8.04) ng/mg Ct, and control group was ( 7.27 ± 5.06 ) ng/mg Ct, respectively ( P ＜0. 05 ). And no significant difference of urinary 8-OHdG levels had been detected among different polymorphism genotypes in TNF-α (G-308A), NF-kB (-94ins/delATTG), iNOS (C608T) and COX-2 (T8473C). Conclusions Urinary 8-OHdG levels in hepatocellular carcinoma tissue are higher than normal. Genetic polymorphisms of NF-kB signaling pathway are not associated with urinary 8-OHdG levels in hepatocellular carcinoma patients.%目的 观察NF-κB信号传导通路相关基因不同表型的肝癌患者尿8-羟基脱氧乌苷(8-OHdG)水平.方法 PCR-RFLP法检测155例肝癌患者(肝癌组)NF-κB信号传导通路中TNF-α、NF-κB、iNOS、COX-2的基因表型.采用ELISA法检测肝癌组及151例健康体检者(对照组)的尿8-OHdG.结果 肝癌组尿8-OHdG水平为(9.29±8.04)ng/mg Cr,对照组为(7.27±5.06)ng/mg Cr,两组相比,P<0.05.肝癌组NF-κB信号传导通路相关基因TNF-α、NF-κB、iNOS、COX-2基因不同表型组尿8-OHdG水平相比,P均>0.05.结论 肝癌患者尿8-OHdG水平高于正常,可能与肝癌发病有关.NF-κB信号传导通路相关基因多态性与肝癌患者尿8-OHdG水平无明显关系.