白藜芦醇对JurcatT细胞分化为Th1/Th2细胞作用的影响及机制

摘要

Objective To approach the effect of resveratrol on the differentiation of Jurcat T cell to Th1/Th2 cell.Methods Jurkat T cells stimulated by phytohemagglutinin (PHA) were treated with different concentrations of resveratrol,and Th1 cell related cytokine IFN-γand Th2 cell related cytokine IL-10 were detected by ELISA, the expression of T-bet and GATA-3 mRNA were detected by RT-PCR.Results The level of IFN-γ,IL-10 and expression of T-bet and GATA-3 mRNA all decreased significantly ,and showed dose and time-dependent manner.Conclusions Resveratrol can inhibit the differentiation of Jurcat T cell to Th1/Th2 cell.%目的 探讨白藜芦醇调节Jurkat T细胞分泌为Th1/Th2型细胞作用的影响.方法 将不同浓度的白藜芦醇作用于受植物血凝素(PHA)刺激的Jurkat T细胞,于不同时间点分别用ELISA法检测Th1型细胞因子IFN-γ和Th2型细胞因子IL-10;RT-PCR法检测T-bet和GATA-3 mRNA表达.结果 白藜芦醇作用后IFN-γ、IL-10及T-bet mRNA、GATA-3 mRNA的表达均明显降低,具有浓度和时间依赖性.结论 白藜芦醇对受PHA刺激的JurcatT细胞由Th0向Th1、Th2细胞分化起抑制作用.