益肾活血汤对糖尿病性ED大鼠阴茎白膜弹性纤维组织的影响

摘要

目的 观察益肾活血汤对糖尿病性阴茎勃起功能障碍(ED)大鼠阴茎白膜弹性纤维组织的影响,探讨其治疗糖尿病性ED的可能机制.方法 取雄性SD大鼠50只,随机取10只为正常组,余40只以链脲佐菌素诱导建立糖尿病模型.行阿朴吗啡阴茎勃起试验筛选糖尿病性ED模型后,按随机化原则选20只并分为模型组、治疗组各10只.治疗组予益肾活血汤药液17.7 g/(kg·d)灌胃,模型组及正常组予等量生理盐水灌胃.给药4周后,在阴茎海绵体勃起状态下取各组阴茎组织,行维多利亚蓝一丽春红染色,彩色图文系统进行分析,观察阴茎白膜弹性纤维累积光密度(IOD)值.结果 治疗组、模型组、正常组阴茎白膜组织中弹性纤维IOD值分别为8.50±2.03、3.96±1.88、9.55±2.67,治疗组明显高于模型组(P＜0.01),与正常组接近(P＞0.05)；模型组显著低于正常组(P＜0.01).结论 益肾活血汤可显著提高糖尿病性ED大鼠阴茎白膜弹性纤维数量；此可能为其治疗糖尿病性ED的可能机制.%Objective To investigate the effect of YiShenHuoXueTang on albuginea penis fibroelastic tissue in diabetic erectile dysfunction rats' in order to reaearch the treating mechanism of YiShenHuoXueTang on diabetic erectile dysfunction. Methods There were 50 male SD rats included in this study. Of all the 50 rats, 10 normal rats were randomly divided into control group and the other 40 rats were induced into diabetic erectile dysfunction model after treated by streptozoto-cin and apomorphine penis erectile test. Twenty diabetic erectile dysfunction rats were chosen randomly and equally divided into two groups, which were diabetic erectile dysfunction group and YiShenHuoXueTang group. Rat s in YiShenHuoXueTang group were treated with YiShenHuoXueTang [17.7 g/(kg · d)] by route of gastric perfusion. Rats in diabetic erectile dysfunction group and control group were treated with normal saline in a same dose and route of administration as YiShenHuoXueTang group. After a drug delivery of 4 weeks, penile tissue of rats in each group was sampled in condition of erection and tested by method of Victoria blue/Ponceau red staining. Integrated optical density (IOD) of elastic fibers in albuginea penis was detected by means of color image analysis. Results IOD of elastic fibers in albuginea penis in YiShenHuoXueTang group, diabetic erectile dysfunction group and control group was (8. 50 ±2. 03), (3.96 ±1. 88), (9.55± 2.61) , respectively. IOD of YiShenHuoXueTang group was higher than that of diabetic erectile dysfunction group (P < 0.01) and closed to control group (P > 0.05). IOD of diabetic erectile dysfunction group was less than that of control group (P<0. 01). Conclusions YiShenHuoXueTang increase the amount of the albuginea penis fibroelastic tissue in rat, which indicates that it relats to the improvement of erectile function of diabetic erectile dysfunction rats.