TROP-2基因小干扰RNA转染对前列腺癌细胞黏附、迁移和侵袭的影响

摘要

目的 观察肿瘤相关钙信号转导蛋白-2( TROP-2)基因小干扰RNA( siRNA)转染对前列腺癌细胞黏附、迁移和侵袭的影响.方法 以TROP-2基因siRNA转染人前列腺癌PC-3细胞后,分别用实时荧光定量PCR和Western blot法检测细胞TROP-2 mRNA和蛋白,用MTT法检测PC-3细胞吸光度值(以此判断细胞黏附力),以Transwell小室实验观察细胞的迁移、侵袭情况.结果 与空载对照组相比,TROP-2 siRNA组TROP-2 mRNA的表达明显下降(P均＜0.01)并呈浓度和时间依赖性(P均＜0.01)；与空白对照组及空载对照组相比,TROP-2 siRNA组TROP-2蛋白的表达明显下降(P均＜0.01)并呈浓度和时间依赖性(P均＜0.01)；与空白对照组相比,TROP-2 siRNA组细胞黏附力下降(P＜0.01)并呈浓度、时间依赖性(P均＜0.05)；与空白对照组及空载对照组相比,TROP-2 siRNA组细胞迁移、侵袭数明显减少(P均＜0.01)并呈浓度依赖性(P均＜0.01).结论 TROP-2基因siRNA转染可抑制前列腺癌PC-3细胞黏附、迁移和侵袭.%Objective To explore the effects of TROP-2 gene small interfering RNA(siRNA)on adhesion, migration, and invasion of human prostate cancer cell. Methods After human prostate cancer PC-3 cell was transfected with different dose of TROP-2 siRNA, the expression of TROP-2 mRNA and protein were were determined by real-time quantitative PCR and Western blot assay, respectively. Cell adhesion was evaluated by MTT assay. The migration and invasion was exmined by Transwell, respectively. Results The results from Real-Time quantitative PCR and Western blot showed that TROP-2 mRNA and protein reduced in time- and dose- dependent manners( all P <0.01). The adhesive, migrative and invasive a-bility of PC-3 cell treated with TROP-2 siRNA decreased compared with control groups ( all P < 0.01). Conclusion TROP-2 gene may play a pivotal role in adhesion, migration, and invasion of human prostate cancer cell. siRNA targeted TROP-2 can inhibit adhesion, migration, and invasion of human prostate cancer cell.