替米沙坦对自发性高血压大鼠反复正加速度暴露后靶器官的影响

摘要

Objective To investigate the effects of telmisartan on the target organ (hearts,brains,kidneys and aortas) of spontaneously hypertensive rats (SHR) after repeated positive acceleration (+ Gz) exposure.Methods Twenty-eight eight-week-old male SHR and fourteen eight-week-old male Wistar Kyoto rats (WKY) were divided into six groups randomly:seven SHR in telmisartan treatment SHR + Gz exposure group(TT-SHR + Gz),seven SHR in telmisartan treatment SHR control group(TT-SHR-C),seven SHR in SHR + Gz exposure group(SHR + Gz),seven SHR in SHR control group(SHR-C),seven WKY in WKY + Gz exposure group(WKY + Gz) and seven WKY in WKY control group(WKY-C).The telmisartan treatment was given to SHR through intragastric administration with 30 mg/(kg · d) telmisartan before and after + Gz exposure to control their blood pressure in normal range.The + Gz exposures were performed with simulating air combat maneuver (SACM) on centrifuge in the profiles of +5,+9,+5,+9,+5 and +9 Gz/10 s with onset rate 1 G/s,the total time was 94 s; and the + Gz exposure was repeated after 20 min rest,for 7 days.The control group rats were not exposed to the + Gz.The specimens of the heart,brain,kidney and aorta of all rats were collected,stained with HE,and observed in light microscopy.Their left ventricular hypertrophy indexes (LVMI) were also measured after the experiment.Results There was no significant differences in the LVMI between TT-SHR-C and WKY-C (P ＞ 0.05),but the LVMI of SHR-C was significantly higher than WKY-C (P ＜0.01).Under light microscopy,the significant histological damage performance was not found in TT-SHR-C and WKY-C; the damage performance of myocardium,kidney and cerebral cortex was found in SHR-C,but that in SHR + Gz was more severe than in SHR-C.Conclusion Telmisartan can effectively reverse early target organ damage of SHR and repeated + Gz exposure does not increase the degree of target organ damage on the condition that the blood pressure is controlled in normal range using telmisartan.%目的 探讨替米沙坦对自发性高血压大鼠(SHR)反复正加速度(+Gz)暴露后靶器官(心、脑、肾、主动脉)的影响.方法 将SHR 28只随机分成4组；Wistar Kyoto大鼠(KY) 14只随机分为2组；每组7只.分别为替米沙坦治疗SHR+ Gz暴露组(TT-SHR+ Gz组)、替米沙坦治疗SHR对照组(TT-SHR-C组)、SHR+ Gz暴露组(SHR+Gz组)、SHR对照组(SHR-C组)、WKY+ Gz暴露组(WKY+ Gz组)、WKY对照组(WKY-C组).替米沙坦治疗SHR在进行+Gz暴露前后给予替米沙坦30 mg/(kg·d)灌胃,将大鼠血压控制在正常范围内.+Gz暴露采取模拟空战动作模式连续进行+5、+9、+5、+9、+5和+9 Gz/10 s暴露,G作用总时间94 s,G增长率为1 G/s.休息20 min后,再按上述模式重复暴露1次,连续暴露7d.对照组不进行离心机暴露.所有大鼠在实验结束后立即采集心、脑、肾、主动脉标本,进行HE染色光镜观察,并对左心室心肌肥厚指数(LVMI)进行测量.结果 替米沙坦治疗SHR的LVMI与WKY无明显差异(P＞0.05),而未服药SHR LVMI明显高于WKY(P ＜0.01).光镜观察替米沙坦治疗SHR组及WKY组大鼠靶器官未见明显组织学损害表现；未服药SHR存在心肌、肾脏、大脑皮层损害表现,SHR+ Gz组损害程度重于SHR-C组.结论 替米沙坦可以有效逆转SHR靶器官早期损害,在血压控制正常基础上进行反复+Gz暴露并不加重SHR靶器官损害程度.