慢性脊髓压迫减压后脊髓缺血再灌注损伤的动物实验研究

摘要

Objective To study weather the existence of reperfusion injury after decompression of chronic spinal cord compression, as well as the relationship between the degree of spinal cord compression and reperfusion injury by use of ani -mal experiments .Methods Nighty-six rabbits were randomly divided into four groups , and each group was divided into two subgroups with 12 rabbits respectively .Group A was the sham operation group , group B ( the percentage of occupying area of the intraspinal lesion was 50%), group C occupying 60%, group D occupying 70%.Group B1, C1 and D1 didn't take the screws out, while group B2, C2 and D2 were removed the screws.And modified Tarlov score, MDA content, GSH-px activity assay , SOD activity assay , TUNEL-positive cell counts were detected after six hours .Results The Tarlov score of group A was significantly higher than that of group B , C and D.The Tarlov score gradually reduced in Group B 1, C1 and D1, and there was a significant difference among these groups (all P<0.05).The Tarlov scale of Group B2 and C2 was higher than that of Group B1 and C1, and there was also a significant difference among these groups .There was a relatively significant difference between Group C1, D1 and Group A1 in MDA content(P<0.05 or <0.01 ).Comparison of the ac-tivity of SOD and GSH-px, there were significantly difference between C1, D1 and A1 (P<0.05 or <0.01 ).Conclu-sions Ischemia-reperfusion injury after decompression of chronic spinal cord compression can occur , and the more severe compression spinal cord endure , the more significant reperfusion injury cause .%目的：探讨慢性脊髓压迫减压后是否存在再灌注损伤，以及脊髓压迫程度与再灌注损伤的关系。方法选择新西兰兔96只，随机分成A、B、C、D 4组，每组又分为2个亚组，每个亚组12只。 A组为假手术组，B组椎管内占位50％，C组为椎管内占位60％，D组为椎管内占位70％。 B1、C1、D1组不取螺钉，B2、C2、D2组取出螺钉后6 h分别进行改良Tarlov评分及MDA含量、GSH-px活性、SOD活性测定，TUNEL阳性细胞计数。结果 Tarlov评分A组显著高于B、C、D组（P均＜0．05）；B1、C1、D1组Tarlov评分依次减低（P均＜0．05），B2、C2组Tarlov评分高于B1、C1组（P均＜0．05）。 C1、D1组与A1组比较，D2组与D1组比较，MDA表达明显增高（P＜0．05或＜0．01）。C1组、D1组与A1组比较，D2组与D1组比较，SOD和GSH-px活性降低明显（P＜0．05或＜0．01）。结论慢性脊髓压迫减压后可以出现缺血再灌注损伤，且脊髓压迫程度越重，再灌注损伤越显著。