系统性红斑狼疮患者外周血白细胞唾液酸转移酶表达变化及机制探讨

摘要

Objective To observe the expression changes of sialyltransferase ( ST8SIA) in the peripheral blood leucocytes of patients with systemic lupus erythematosus ( SLE) and to investigate the possible mechanism.Methods ST8SIA1-6 was detected by RT-PCR in the peripheral blood leucocytes in 24 SLE patients and 24 normal donors. We analyzed the miRNAs that targeting 3`untranslated region ( UTR) by microarray chip.Results The expression of ST8 SIA2 , ST8 SIA3 and ST8 SIA5 was not detected in the peripheral blood leucocytes of the two groups .The relative expression levels of ST8SIA1 mRNA, ST8SIA4 mRNA and ST8SIA6 mRNA in the observation group were 1.524 ± 0.229, 13.78 ±1.669 and 2.238 ±0.387, and they were 1.129 ±0.106, 1.087 ±0.089 and 0.550 ±0.054 in the control group.The difference in ST8 SIA4 mRNA and ST8 SIA6 mRNA of the two groups was statistically different ( all P<0.05).There were 12 down-regulated plasma miRNAs that regulated the 3`UTR of ST8SIA4 mRNA and 1 up-regulated plasma miRNA.There were 4 plasma miRNAs that made the 3`UTR of ST8SIA6 mRNA down-regulated and 4 plasma miRNAs up-regulated.Conclusion The expression levels of ST8SIA4 mRNA and ST8SIA6 mRNA in patients with SLE are increased, which may be related with the abnormal expression of plasma miRNAs that can regu-late the 3`UTR of ST8SIA4 mRNA and ST8SIA6 mRNA.%目的：观察系统性红斑狼疮（SLE）患者外周血白细胞唾液酸转移酶（ST8SIA）的表达变化，并探讨其机制。方法选择SLE患者（观察组）和健康志愿者（对照组）各24例，采用RT-PCR法检测外周血白细胞ST8SIA1～6 mRNA；对有统计学意义的ST8SIA mRNA，采用基因芯片技术分析血浆中靶向其3′非翻译区（3′UTR）的miR-NA。结果两组外周血白细胞均无ST8SIA2、ST8SIA3、ST8SIA5 mRNA表达；观察组ST8SIA1、4、6 mRNA相对表达量分别为1．524±0．229、13．780±1．669、2．238±0．387，对照组分别为1．129±0．106、1．087±0．089、0．550±0．054；两组ST8SIA4、6 mRNA比较，P均＜0．05。靶向调节ST8SIA4 mRNA的miRNA表达下降12条、升高1条，靶向调节ST8SIA6 mRNA的miRNA表达下降、升高各4条。结论 SLE患者外周血白细胞中ST8SIA4、ST8SIA6 mR-NA表达升高，与对其进行靶向调控的miRNA表达异常有关。