病变组织细胞免疫组织化学 P63／SMA 双标记对乳腺癌的诊断效能

摘要

Objective To investigate the diagnostic efficiency of P63/smooth muscle actin ( SMA) double labeled im-munochemistry in lesion tissues of breast cancer.Methods A total of 150 cases of patients with breast disease were select-ed as the subjects.Needle aspiration was performed to attain the breast specimens.HE staining and double labeled immu-nohistochemical staining of P63 and SMA were used.The results were observed and analyzed by using light microscope. Results Both P63 and SMA were positive in the vast majority of breast myoepithelial cells and P63 was negative in non-myoepithelial cells.Thirty-two cases by HE staining and 20 cases by double labeled immunohistochemical staining were di-agnosed with benign breast lesions, respectively;and 119 cases by HE staining and 121 cases by double labeled immuno-histochemical staining of suspicious carcinoma cells or carcinoma cell were found.When we compared the pathological re-sults, the accuracy rates in diagnosis of benign breast lesions with HE staining and double labeled immunohistochemical staining were 91%and 97%, respectively.The diagnostic accuracy rates of malignant breast lesions were 100% by both HE and double labeled immunohistochemical staining.Conclusion P63 and SMA double labeled immunohistochemical staining has higher accuracy rate in diagnosis of breast cancer and it could effectively diagnose the benign breast lesions.%目的：探讨P63／平滑肌肌动蛋白（ SMA）双标记法免疫组化检查对乳腺癌诊断的效能。方法体检发现乳腺肿块的患者150例，行乳腺针吸检查取病变组织，分别进行HE染色和P63／SMA双标记法免疫组化染色，光镜下观察结果并分析。结果乳腺肌上皮细胞同时表达P63和SMA，非肌上皮细胞均不表达P63。 HE染色、P63／SMA双标记法免疫组化染色检查分别有32例、20例初诊报告为乳腺良性病变，分别有119例、121例初诊报告为可疑癌细胞或发现癌细胞。对比患者手术病理结果，HE染色对乳腺良性病变的诊断准确率为91％，P63／SMA双标记法免疫组化检查为97％；HE染色和P63／SMA双标记法免疫组化检查对乳腺恶性病变的诊断准确率均为100％。结论 P63／SMA双标记法免疫组化检查对乳腺癌的确诊率较高，且较HE染色可更好地鉴别乳腺良性病变。