骨髓间充质干细胞旁分泌效应对胰腺β细胞增殖、凋亡及分泌功能的影响

摘要

Objective To investigate the influence of paracrine effects of bone mesenchymal stem cells (BMSCs)on the proliferation,apoptosis and insulin secretion of pancreaticβcell line INS-1 cells.Methods The BMSCs were cultured and BM-SCs conditioned media (BMSCs-CM)were obtained by culturing BMSCs with rat pancreatic extracts (RPE)which simulated the pancreatic microenvironment.The INS-1 cells were divided into groups A,B and C,which were cultured with BMSCs-CM,con-ditioned media without RPE and RPMI 1640 culture solution,respectively.The influence of BMSCs-CM on INS-1 cell prolifera-tion was analyzed by MTT.The cell proliferation rate was calculated.The effects of BMSCs-CMon the INS-1 cell apoptosis were measured by AnnexinⅤ/PI double staining.The rate of apoptosis was counted.The insulin concentration of INS-1 cells was ana-lyzed by radioimmunoassay (RIA).Results The cell proliferation rate in the group A was higher than that in group B and C (P<0.05).The apoptosis rate in the group A was lower than that in group B and C (P<0.05).The insulin concentration in the group A was higher than that in group B and C at the concentration of 5.6 mmol/L and 20.0 mmol/L (all P<0.05).Conclu-sions The incubation of BMSCs-CMcan stimulate the INS-1 cell proliferation,inhibit the apoptosis,and promote the insulin se-cretion.The above actions of BMSCs may be achieved by paracrine effects.%目的：观察骨髓间充质干细胞（BMSCs）旁分泌效应对胰腺β细胞系INS-1细胞增殖、凋亡及胰岛素分泌的影响。方法培养BMSCs，以胰腺提取物（RPE）模拟胰腺微环境培养BMSCs后，获取BMSCs的条件培养液（BMSCs-CM）。将细胞分为A组、B组、C组，分别以BMSCs-CM、不含RPE的条件培养液、等量RPMI 1640培养液培养。采用MTT法观察各组细胞增殖情况，计算细胞增殖率。采用AnnexinⅤ／PI法观察各组细胞凋亡情况，计算细胞凋亡率。应用放射免疫法（RIA）测定各组胰岛素水平。结果 A组细胞增殖率高于 B 组及 C 组（P均＜0．05）。A组细胞凋亡率低于B组及C组（P均＜0．05）。在葡萄糖溶液终浓度为5．6及20．0 mmol／L时，A组胰岛素水平均高于B组及C组（P均＜0．05）。结论 BMSCs-CM培养INS-1细胞后可促进其增殖、抑制其凋亡并促进其分泌胰岛素，BMSCs可能通过旁分泌效应发挥以上作用。