食管鳞癌组织中Wnt2、Dvl mRNA和蛋白表达变化及意义

摘要

Objective To observe the expression changes of Wnt2 and dishevelled ( Dvl) mRNA and protein in the esophageal squamous-cell carcinoma ( ESCC) tissues, and to investigate the significance.Methods Sixty cases of tumor tissue specimens ( observation group) and adjacent tissues ( control group) from ESCC patients were selected.The mRNA and protein expression of Wnt2 and Dvl in the two groups was respectively detected by RT-PCR and Western blotting.Re-sults In the observation group, the mRNA expression of Wnt2 and Dvl was respectively 1.007 ±0.237 and 1.008 ± 0.220, the protein expression of Wnt2 and Dvl was respectively 0.512 ±0.406 and 1.218 ±1.082, and that in the control group was respectively 0.877 ±0.119, 0.891 ±0.196, 0.153 ±0.189 and 0.505 ±0.358.The mRNA and protein ex-pression of Wnt2 and Dvl in the observation group was higher than that of the control group ( all P<0.05) .In the observa-tion group, Wnt2 and Dvl mRNA was positively correlated with each other (r=0.807, P<0.01).Conclusion The high expression of Wnt2 and Dvl gene is found in ESCC tissues, which indicates Wnt signaling pathway plays an important role in the occurrence and development of ESCC.%目的 观察食管鳞癌组织中Wnt2、散乱蛋白(Dvl)mRNA和蛋白的表达变化,并探讨其意义.方法 取60例食管鳞癌患者肿瘤组织标本为观察组,癌旁组织标本为对照组.用RT-PCR法检测两组Wnt2、Dvl mRNA相对表达量;用Western blot法检测两组Wnt2和Dvl蛋白表达量.结果 观察组Wnt2 mRNA、Dvl mRNA、Wnt2蛋白、Dvl蛋白表达量分别为1.007±0.237、1.008±0.220、0.512±0.406、1.218±1.082,对照组分别为0.877±0.119、0.891±0.196、0.153±0.189、0.505±0.358,观察组Wnt2 mRNA、Dvl mRNA、Wnt2蛋白、Dvl蛋白表达量均高于对照组,P均<0.05.观察组Wnt2与Dvl mRNA表达呈正相关(r=0.807,P<0.01).结论 食管鳞癌组织中存在Wnt2及其通路关键蛋白Dvl mRNA及蛋白的高表达.Wnt信号通路在食管鳞癌的发生、发展中发挥了重要作用.